Bioinformatics predictions of immune mechanism and traditional Chinese medicine of multi-brain region of Alzheimer's Disease
Objective To identify differentially expressed genes(DEGs)in different brain regions of Alzheimer's disease(AD)using bioinformatics techniques,explore the immune mechanisms in each brain region,and predict potential therapeutic drugs.Methods AD sample data were obtained from the GEO database,and DEGs analysis and weighted gene co-expression network analysis(WGCNA)were performed on each brain region.The core genes in the most related modules were obtained by protein-protein interaction(PPI)network analysis,and their diagnostic value was evaluated by ROC curve.Differential genes were subjected to immune pathway enrichment analysis,and immune cell infiltration patterns were analyzed using C1BERSORT algorithm.Potential therapeutic drugs for AD were screened using Coremine Medical.Results A total of 3 280 differential genes were identified in the EC region,1 591 in the HIP region,3 995 in the MTG region,2 056 in the PC region,907 in the SFG region,and 1 480 in the VCX region.EC and VCX were highly related to the blue module,HIP and PC were highly related to the turquoise module,and MTG and SFG were highly related to the green and yellow modules.The PPI network showed that there were four Hub genes in the EC region,13 in the HIP region,four in the MTG region,nine in the PC region,and 17 in the SFG region.Different brain regions were enriched with different immune pathway modules.Subsequently,Curcumae Longae Rhizoma was identified as a potential traditional Chinese medicine(TCM)for AD treatment based on its association with key genes such as EP300,PPARG,CND1,GSK3B,BCL2,EGFR,KDR,MYC,and 1L1B.Conclusion AD patients exhibit diverse immune pathways across distinct brain regions,and this disparity is intricately associated with the pathogenesis of AD.Curcumae Longae Rhizoma holds promising potential as a Chinese medicinal intervention for the treatment of AD.
Alzheimer's diseasebioinformaticsscreening of traditional Chinese medicinesimmune cell infiltrationCurcumae Longae Rhizoma
NETL
NSTL
万方数据
