Effect of Feire Puqing Powder against hepatitis coronavirus MHV-A59 infection and inflammatory factors in mice
Objective To explore the effects and inflammatory factors of the Tibetan medicine Feire Puqing Powder(FPP)on the mouse model of hepatitis coronavirus A59(MHV-A59)infection.Methods Male Kunming mice were used as experimental subjects treated with MHV-A59 by nose-dropping,and six groups were set up:the control,the model,the positive(ribavirin),and the FPP's low-,medium-,and high-dosage(25,50,and 100 mg·kg-1)groups,and the FPP was ig administered once a day for 10 consecutive days,and the body weight of the mice during the modeling period was examined and recorded,and the lung and liver indices of the mice were examined after execution;The serum levels of IL-1β and IL-18 were determined using the Elisa kit;The mRNA of IL-1βand IL-18 in the liver,and the viral load in the serum were detected using the qRT-PCR;The pathological sections of liver and lung were stained with hematoxylin-eosin(HE),and the necrotic and inflammatory cell infiltration of the liver and lung in each experimental group were observed and recorded under the microscope.Results Compared with the control group,the body weight of the model group increased slowly(P＜0.01);The body weight of the three dose groups of FPP increased daily,and there was a significant difference between middle and high dose groups with the model group on days 5-8(P＜0.01);There was a significant difference at the liver index of the middle dose group of FPP vs the model group(P＜0.05),while others were no significant difference on the lung index of the mice in each group;The serum inflammatory factors IL-1β and IL-18 were significantly elevated(P＜0.001),and compared with the model group,all three dose groups of FPP reduced the serum level of IL-18,(P＜0.001,0.01);The IL-1β and IL-18 were significantly elevated in liver tissues of the model group(P＜0.001);Compared with the model group,the transcript level of IL-1β in the liver treated by FPP high-dose group was significantly reduced(P＜0.01);The level of IL-18 in the liver tissues of experimental animals in FPP triple-dose group did not show significant changes;The serum MHV load in each group of mice showed that,compared with the model group,there was a significant reduction of MHV load in the serum in the ribavirin group and the FPP high-dose group(P＜0.01);Liver histopathological sections showed that:Model group showed extensive necrosis and degeneration,with inflammatory cell infiltration and ballooning,while punctate foci and inflammatory cell infiltration were reduced in the FPP high-dose and ribavirin groups;Histopathological sections of lung tissue showed that:lung tissue of the model group showed obvious thickening of alveolar septa,mesenchymal edema,compressed alveoli,with inflammatory cell infiltration,and capillary dilation and congestion,capillary dilatation and congestion,while the alveolar intervals of lung tissues in the middle-dose group,high-dose group and ribavirin group of FPP were narrower,the alveoli were stretched out,and there were still inflammatory infiltration and hemorrhage locally,and secretion in the alveolar lumen was significantly reduced.Conclusion FPP has a protective effect on the MHV-A59 mouse infection,and the mechanism is related to the inhibition of viral replication and the inhibition of inflammatory factor increase.
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