灯盏花乙素通过PPARγ-PGC1α-UCP1通路改善小鼠肥胖作用及机制
Scutellarin ameliorates obesity in high-fat fed mice by regulating PPARγ-PGC1α-UCP1 signaling pathway
张琦 1田冲冲 1张洋 1方晨曦 1卜薇 1李凤1
作者信息
- 1. 江苏医药职业学院药学院,江苏盐城 224005
- 折叠
摘要
目的 探究灯盏花乙素对肥胖小鼠的干预作用及可能的分子机制,为肥胖的治疗提供借鉴.方法 采用高脂膳食构建小鼠肥胖模型,并采用灯盏花乙素(400mg·kg-1)干预8周,测定各组小鼠肥胖相关指标,包括体质量变化、脂肪组织质量、血脂指标[总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)及高密度脂蛋白胆固醇(HDL-C)]等;采用苏木精-伊红(HE)染色法分析脂肪组织形态的改变;分别采用免疫荧光方法、实时荧光定量PCR(qRT-PCR)及Western blotting法检测脂肪组织中脂质代谢相关基因[过氧化酶体增殖物激活受体γ(PPARγ)、PPARγ共激活因子1α(PGC1α)与解偶联蛋白1(UCP1)]、蛋白(PPARγ、PGC1α及UCP-1)表达水平的变化.结果 结果显示,灯盏花乙素干预可显著降低高脂喂养小鼠的体质量及脂肪组织质量(P＜0.05、0.01);另外,灯盏花乙素可改善血脂指标——TC、TG、LDL-C及HDL-C;组织病理学结果显示,灯盏花乙素可以减轻脂肪组织中的脂质沉积.进一步qRT-PCR结果显示,灯盏花乙素极显著提高肥胖小鼠脂肪组织中PPARγ的mRNA表达水平(P＜0.05),同时还能升高PGC1α与UCP1的mRNA表达水平,差异具有极显著性(P＜0.01).Western blotting结果则进一步验证了灯盏花乙素能显著提高高脂喂养小鼠脂肪组织PPARγ、PGC1α以及UCP1的蛋白表达水平(P＜0.05).结论 灯盏花乙素可以改善高脂喂养小鼠肥胖,该作用可能与激活PPARγ-PGC1α-UCP1信号通路,调节脂肪组织的能量代谢有关.
Abstract
To explore the intervention effect of scutellarin(SCU)on obese mice fed with high-fat diet and its possible molecular mechanism,and provide reference for the treatment of obesity.Firstly,a mouse obesity model was constructed with a high-fat diet,and SCU was used to intervene for 8 weeks.Obesity-related indexes,such as weight change,adipose tissue weight and lipid indexes,were measured in each group.The changes of adipose tissue morphology were analyzed by HE staining.Immunofluorescence,quantitative real-time fluorescence polymerase chain reaction(qRT-PCR)and Western blotting were used to detect the expression levels of lipid metabolism-related genes and proteins in adipose tissue.The results showed that the body weight and adipose tissue weight of high-fat fed mice could be significantly reduced after SCU intervention.In addition,SCU could improve total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)and high density lipoprotein cholesterol(HDL-C)(P＜0.05,0.01).Histopathological results showed that SCU could reduce lipid deposition in adipose tissue.Further,qRT-PCR results showed that SCU significantly increased the mRNA expression level of peroxidase proliferator-activated receptor γ(PPARγ)in adipose tissue of high-fat fed mice(P＜0.01),and significantly increased the mRNA expression level of PGC1α and uncoupling protein-1(UCP1)in high-fat fed mice(P＜0.01).Western blotting results further demonstrated that SCU significantly increased the protein expression levels of PPARγ,PGC1α and UCP1 in adipose tissue of high-fat fed mice(P＜0.01).Conclusion SCU can effectively improve obesity in high-fat fed mice,and the improvement may be related to the PPARγ-PGC1α-UCP1 signaling pathway.
关键词
灯盏花乙素/肥胖/PPARγ-PGC1α-UCP1信号通路/血脂/脂质代谢Key words
scutellarin/obesity/PPARγ-PGC1α-UCP1 signaling pathway/blood lipid/lipid metablishment引用本文复制引用
基金项目
江苏省盐城市卫生健康委员会2023年度医学科研立项项目(YK2023032)
江苏省盐城市卫生健康委员会2023年度医学科研立项项目(YK2023097)
江苏高校哲学社会科学研究一般项目(2022SJYB2075)
江苏省高职院校青年教师企业实践计划(2023QYSJ047)
江苏医药职业学院自然科学基金研究重点项目(20214107)
江苏医药职业学院自然科学基金研究重点项目(20214103)
江苏医药职业学院校本教育教学研究课题(Q202303)
江苏医药职业学院校本教育教学研究课题(Y202322)
出版年
2024
NETL
NSTL
万方数据