Eucalyptol,Limonene and Pinene Enteric Soft Capsules relieve otitis media with effusion in mice based on AQP5-TLR4/MyD88/NF-κB pathway
Objective To investigate the therapeutic effect and mechanism of Eucalyptol,Limonene and Pinene Enteric Soft Capsules on otitis media with effusion(OME)model in mice based on aquaporin 5(AQP5)-Toll-like receptor 4(TLR4)/myeloid differentiation factor 88(MyD88)/nuclear transcription factor κB(NF-κB)pathway.Method Kunming mice were randomly divided into control group,model group,dexamethasone group,low-,medium-and high-dose(55,110,and 220 mg·kg-1)groups of Eucalyptol,Limonene and Pinene Enteric Soft Capsules.The mice OME model was established by injecting LPS through the tympanic membrane.The structural changes of tympanic membrane in each group were observed microscopically.Hematoxylin-eosin(HE)staining was used to assess the histopathological changes of the middle ear.ELISA was used to detect the levels of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in the middle ear lavage fluid.Western blotting was used to detect the expression of AQP5 and TLR4/MyD88/NF-κB signaling pathway-related proteins in the middle ear mucosa.Results Compared with the control group,the tympanic empyema,tympanic mucosa thickening,inflammatory cell infiltration,IL-6 and TNF-α levels in the middle ear lavage fluid were considerably increased(P＜0.01),AQP5 protein expression was significantly decreased(P＜0.01),and TLR4,MyD88,p-IκBα,and p-p65 protein expression was significantly increased(P＜0.01)in the model group.Compared with the model group,each dose of Eucalyptol,Limonene and Pinene Enteric Soft Capsules could reduce tympanic empyema,reduce inflammatory cell infiltration,considerably reduce IL-6 and TNF-α levels in middle ear lavage fluid(P＜0.01),significantly increase AQP5 protein expression(P＜0.01),and significantly reduce TLR4,MyD88,p-IκBα,and p-p65 protein expression(P＜0.01).Conclusion Eucalyptol,Limonene and Pinene Enteric Soft Capsules can reduce LPS-induced OME by increasing the expression of AQP5 protein and inhibiting the activation of TLR4/MyD88/NF-κB signaling pathway.
Eucalyptol,Limonene and Pinene Enteric Soft Capsulesotitis media with effusionLPSAQP5TLR4/MyD88/NF-κB pathway
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