Protective effect of Modified Yiwei Decoction on gastric ulcer induced by alcohol in rats
Objective To evaluate the protective effect of Modified Yiwei Decoction on ethanol-induced gastric ulcer model rats.Methods SD rats were randomly divided into control group,model group and Modified Yiwei Decoction low-,medium-and high-dose group(0.8,1.2 and 1.7 g·kg-1),eight rats in each group.The drugs were given by ig administration before the model was established,once a day for 30 d,all the rats were strictly abstinence for 24 h(water freedom).Excluding rats in the control group,the rest of the rats were given 1 mL of absolute ethanol by ig administration to induce gastric ulcers.One hour later,all rats were sacrificed immediately.The gastric mucosal surface was unfolded to take pictures and the score of gastric damage was measured.HE staining of stomach sections were carried out to observe pathological changes.Myeloperoxidase(MPO),total superoxide dismutase(SOD),prostaglandin E2(PGE2),pepsinogen Ⅰ(PGⅠ),pepsinogen Ⅱ(PGⅡ),vascular endothelial growth factor(VEGF),interleukin-6(IL-6),interleukin-1β(IL-1 β)and tumor necrosis factor-α(TNF-α)were detected.In addition,human gastric mucosal epithelial cells(GES-1)and human umbilical vein endothelial cells(HUVEC)were cultured in vitro,and the effects of Modified Yiwei Decoction on proliferation and migration were evaluated by MTT and scratch tests.Results Modified Yiwei Decoction can significantly reduce the damage of gastric mucosa and the pathological score of gastric mucosa(P＜0.001).Gastric MPO activity was significantly decreased and serum SOD activity was increased(P＜0.05,0.001).The contents of PGE2,PGⅠ and PGⅡ in stomach were significantly increased(P＜0.05,0.001).The contents of TNF-α,IL-1β and IL-6 in serum were significantly decreased(P＜0.01,0.001).And increased serum VEGF level(P＜0.05)Promote proliferation and migration of GES-1 and HUVEC cells(P＜0.01,0.001).Conclusion Modified Yiwei Decoction can inhibit the secretion of inflammatory factors,relieve oxidative stress,protect gastric mucosa,promote cell proliferation and migration,and play a protective role in gastric ulcer model rats.
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