Analysis of prescription rules and mechanism of action of core drug pairs in Puji Fang for rheumatoid arthritis
Objective Through data mining,the medication rules and prescription characteristics of relevant prescriptions for the treatment of rheumatoid arthritis(RA)in Puji Fang were analyzed,and the mechanism of action of core drugs against RA was analyzed by network pharmacology.Methods The prescriptions for RA in Puji Fang were collected by searching the electronic book system of Chinese Medical Dictionary,and Excel software was used to calculate the properties,tastes,meridian-tropism and efficacy.The frequency,association rules and clustering rules of traditional Chinese medicine were analyzed by R language.Based on the data mining results,the core drug pair was obtained,and the Chinese Medicine System Pharmacology Database and Analysis Platform(TCMSP),Pharmmapper,String and other databases were used to construct the"drug-component-target-disease"and protein-protein interaction(PPI)network using R language and Cytoscape software.Functional enrichment analysis of core drugs against RA targets was performed.Results A total of 601 prescriptions for RA and 321 Chinese herbs were collected in Puji Fang.High frequency Chinese herbs(Cinnamomi Cortex,Saposhnikoviae Radix,Aconiti Lateralis Radix Praeparata,Angelicae Sinensis Radix,Glycyrrhizae Radix et Rhizoma,Chuanxiong Rhizoma,Notopterygii Rhizoma et Radix,etc.)with frequency ≥ 40 were mainly warm and spicy,and mostly affected the spleen and kidney meridians.The main effects were supplementing deficiency,relieving external symptoms and warming the interior.Seventeen groups of drug pairs were obtained by high-frequency Chinese medicine association rules(with the highest confidence of"Angelicae Pubescentis Radix-Saposhnikoviae Radix"as the core drug pair),and four groups of cluster prescriptions were obtained by systematic clustering.A total of 24 active ingredients,306 targets and 3 964 RA-related targets were obtained from each database,and 173 intersection targets were generated.Network analysis results showed that angelicone,mandenol,angelol D,sitosterol,etc.may be the main active ingredients of anti-RA,and the key targets were ALB,HSP90AA1,MMP9,EGFR,etc.It may play a role in anti-RA by regulating Ras signaling pathway,P13K-Akt signaling pathway,FoXO signaling pathway and MAPK signaling pathway.Conclusion The traditional Chinese medicines in Puji Fang for RA are mainly used for warming and xin,regulating spleen and kidney and tonifying deficiency.The results of network pharmacology show that the core drugs may play a therapeutic role in"Angelicae Pubescentis Radix-Saposhnikoviae Radix"by regulating multiple targets and pathways.
rheumatoid arthritisPuji Fangdata miningcore drug pairsnetwork pharmacologyAngelicae Pubescentis Radix-Saposhnikoviae Radixangeliconemandenolangelol D
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