Preparation of resveratrol temperature sensitive gel for chemotherapy via bladder perfusion
Objective To prepare resveratrol temperature-sensitive gel for the treatment of bladder cancer,which can be administered by bladder perfusion.Methods Using the lowest gelation temperature and dissolution time as indicators,the optimal gel formula was selected,the microscopic structure of the gel was observed by scanning electron microscope(SEM),and the compatibility of the gel matrix with the drug was observed by Fourier transform infrared spectroscopy(FTIR),the in vitro release behavior of the gel was examined by dissolution tester,the migration and proliferation of MB49-LUC mouse bladder cancer cells were examined by scratch test and MTT assay with resveratrol concentration and action time as variables.Results The optimal gel formula was resveratrol 30％,polyoxyethylene sorbitan monolaurate 407 19％,polyoxyethylene sorbitan monooleate 188 7％,alginic acid 0.3％,calcium carbonate 0.06％,and ammonium bicarbonate 0.2％,SEM results showed that the gel had a loose and porous three-dimensional network structure,FTIR showed no significant change in the functional group structure of the gel material,indicating good compatibility,the drug was slowly released from the floating gel,with a cumulative release of 50.37％within 80 days,and a total drug release of 135.58 mg.The scratch test showed that compared with the control group,resveratrol concentrations of≥60 pmol·L-1 could significantly inhibit themigration of MB49-LUC cells(P＜0.05),the MTT assay showed that compared with the control group,the 120,150 μmol·L-1 groups at 24 h of resveratrol action,the 60,90,120,150 μmol·L-1 groups at 48 h,the 30,60,90,120,150 pmol L-1 groups at 72 h,and the 15,30,60,90,120,150 μmol·L-1 groups at 96,120 h had significantly lower cell viability(P＜0.01,0.001).Conclusion The inhibitory effect of resveratrol on bladder cancer cells is dose-dependent and time-dependent.With the long-lasting drug release capacity of temperature-sensitive gel,resveratrol temperature-sensitive gel has obvious inhibitory effect on bladder cancer cells.
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