Objective To study the absorption differences of danshensu,neochlorogenic acid,protocatechualdehyde,chlorogenic acid,cryptochlorogenic acid,caffeic acid,rosmarinic acid,and salvianolic acid B in Huamoyan Granules in different parts of the small intestine(duodenum,jejunum and ileum),and to investigate the intestinal absorption characteristics of the main components of Huamoyan granules.Methods An in situ single-pass intestinal perfusion method was established,and the concentrations of the above eight index components were detected by high performance liquid chromatography(HPLC);the absorption of the eight index components in different intestinal segments was investigated,and the intestinal absorption parameters were calculated.Results The apparent permeability coefficients(Papp)of the eight index ingredients were all greater than 1.2 × 10-3 cm·min-1,indicating that the absorption of each ingredient was good in different intestinal segments;the absorption of each ingredient was best in the jejunum,followed by the ileum and duodenum.Conclusion The absorption of each index ingredient in Huamoyan preparation in the small intestine was good,which was in line with the requirements of oral preparation,and also provided an objective basis for exploring the study of the main pharmacodynamic components of Huamoyan preparation and their action characteristics.
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