Pharmacodynamic study on intervention of Yanshikang Granules in rat model of pediatric anorexia induced by special forage and tail clipping
Objective To investigate the therapeutic effect of Yanshikang Granules in a rat model of pediatric anorexia induced by a special forage and tail clipping.Methods A rat model of pediatric anorexia nervosa was established using a special chow stacked on a tail-clamped young rat.The rats were randomly divided into six groups,including control group,model group,Yanshikang Granules low,medium,high dose(2.8,5.6,11.1 g·kg-1)groups,and Erzhiling Chongji(10.1 g·kg-1)group.The general status,body weight and food intake of each group of rats were monitored,and the frequency and amplitude of the duodenal bioelectrical signal was detected by MP 150 system,serum levels of leptin(LEP),somatostatin(SST),motilin(MTL),and gastrin(GAS)were measured by ELISA method,the structure of gut microbial community were measured by 16S RNA high-throughput sequencing technology.Results Compared with the model group,after treatment with Yanshikang Granules,food intake and body weight were significantly improved(P＜0.05,0.01),amplitude and frequency of duodenal bioelectrical signal were significantly improved(P＜0.05,0.01),serum LEP and SST were significantly reduced(P＜0.05,0.01,0.001),serum MTL were significantly improved(P＜0.05,0.01),the abundance of Blautia were significantly reduced(P＜0.05).Conclusion Yanshikang Granules can treat the anorexia situation in a rat model of anorexia caused by the combination of special forage and tail clamping.The mechanism is related to regulating the secretion of gastrointestinal hormone and improving the gut microbial environment.
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