Effects of alcohol extract of clove at 39 ℃ on proliferation,migration and apoptosis of oral squamous cell carcinoma
Objective To investigate the effects of alcohol extract of clove(AEC)combined with 39 ℃ on the proliferation,migration and apoptosis of oral squamous cell carcinoma(OSCC).Methods Oral squamous cells(OSC)were cultured with 0,0.05％,0.10％,or 0.20％AEC at 39 ℃ for 72 h.CCK-8 and colony formation assays were used to detect the proliferation of OSCC.The migration ability of cells was detected by scratch test.The apoptosis of the cells was detected by flow cytometry and Hoechest 33342 staining.Results Compared with the control and model group,the 0.05％,0.10％and 0.20％groups showed a gradual decrease in the proliferation activity of OSC.With the increase of the concentration of 0.05％,0.10％,and 0.20％groups,the colony formation decreased,and the cell migration and invasion ability gradually decreased.The apoptosis rate of OSC increased gradually,both in a concentration dependent manner with AEC.Combined with 39 ℃ treatment,the inhibition of proliferation,migration and invasion of OSC was more obvious,and cell apoptosis was more significant.Conclusion AEC can inhibit the proliferation and migration of OSCC,and promote the apoptosis of OSCC.When used in combination with 39 ℃,it has a synergistic effect,so as to achieve the therapeutic effect of OSCC.
alcohol extract of cloveoral squamous cell carcinomaproliferationmigrationapoptosisthermotherapy
万方数据
