Advance in establishment and application of patient-derived tumor xenograft model
Patient-derived tumor xenograft(PDX)model is an in vivo tumor model implanting human tumor tissues or patient-derived primary cells into immunodeficient mice,and then culturing the cells in passaging.PDX model is derived from clinical patients and can interact with microenvironment in mice.As an animal model,PDX model can largely restore the characteristics of human tumors,thus it is widely used in preclinical pharmacodynamic evaluation of anti-tumor drugs,personalized medical treatment,as well as research on the distribution and metastatic mechanism of tumors,etc.The success of PDX model establishment is highly correlated with tumors'malignancy and freshness,as well as immunodeficiency in mice.It's a fact that only few new anti-cancer drugs are approved by the regulatory authorities each year.Due to the lack of standardized preclinical evaluation models and system,the research and development process of antitumor drugs in China has been seriously limited,thus it is imperative to establish reasonable and reliable preclinical efficacy evaluation models and a scientific evaluation technology system for anti-tumor drugs.This paper will review the establishment and application of PDX model,with a view to providing reference for establishing and applicating effective new models for the preclinical evaluation of anti-tumor drugs in China.
patient-derived tumor xenograftanti-tumor drugpharmacodynamic evaluation of drugsindividualized treatmentmetastatic mechanism of tumor
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