Study on metabolites and metabolic pathways of Pseudostellaria heterophylla cyclic peptides heterophyllin A and pseudostellaria C in rats by UPLC-Q-Exactive MS technology
Objective To analyze and identify the metabolites present in plasma,bile,urine and feces after gavage of the cyclic peptide extract of Pseudostellaria heterophylla and to speculate on the metabolic pathways of different structural cyclic peptides in rats.Methods Male SD rats were ig administered cyclic peptide extract(400 mg·kg-1),and plasma,bile,urine and feces samples were collected.After the samples were processed,the metabolites of two of the blood entry cyclic peptides,heterophyllin A(HA)and pseudostellaria C(PC)were structurally characterized by ultra-high-performance liquid chromatography-quadrupole-electrostatic field orbital ion trap mass spectrometry(UPLC-Q-Exactive MS).Results Seven cyclic peptide components were extracted from plasma,including pseudostellarin A,pseudostellarin B,heterophyllin B,PC,pseudostellarin D,HA,and pseudostellarin E.13 metabolites of HA and 15 metabolites of PC were detected in biological samples from rats.3,7,0,and 13 of HA metabolites and 3,10,4,and 14 of PC metabolites were identified in plasma,bile,urine,and feces,respectively,and the metabolism of the two cyclic peptides in rats was elucidated through the pathways of dehydroxylation,hydrolysis,dehydration,and methylation.Conclusion The metabolites of HA and PC were deduced by UPLC-Q-Exactive MS.
Pseudostellaria heterophylla cyclic peptidesultra-high-performance liquid chromatography-quadrupole-electrostatic field orbital ion trap mass spectrometryheterophyllin Apseudostellaria Cmetabolitesmetabolic pathway
赵立、阚永军、蒋畅、庞文生、胡娟
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福建省中医药科学院药物研究所,福建 福州 350003
福建中医药大学药学院,福建 福州 350122
福建中医药大学附属第二人民医院药学部,福建 福州 350003
太子参环肽 超高效液相色谱-四极杆-静电场轨道离子阱质谱技术 heterophyllin A pseudostellaria C 代谢产物 代谢途径
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