Objective Establishment of a new malignant lymphoma model of thymus in p53+、-mice induced by N-methyl-N-nitrosourea(MNU).Methods Administration of 75 mg·kg-1 MNU to p53+/-mice via a single ip,followed by general symptom observation,food intake,and body mass measurement after administration.After 10 weeks of treatment with MNU,the levels of serum alkaline phosphatase(ALP)and lactate dehydrogenase(LDH)were measured using a fully automated biochemical analyzer;Perform gross examination after dissection,and perform histopathological examination of thymus tissue after HE staining;Immunohistochemical detection of CD20,CD3,CD68 expression in thymus.Results Compared with the control group,the food intake and body weight of p53+/-mice decreased(P＜0.05,0.01),and symptoms such as emaciation,hunchback,and shortness of breath were observed in weeks 9-10,even leading to animal death.Serum ALP significantly decreased(P＜0.01)and LDH significantly increased(P＜0.01).Gross pathological examination showed an increase in thymus volume,appearing pale yellow white,and microscopic examination revealed malignant lymphoma of the thymus;Immunohistochemical detection showed positive expression of CD3 and CD68 in thymic malignant lymphoma,while CD20 showed negative expression.Conclusion Single intraperitoneal injection of 75 mg·kg-1 MNU can induce thymus malignant lymphoma in p53+/-knockout mice,and the tumor was derived from T cells and the morphological and immunological characteristics were similar to human T-LBL.
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