Analysis of color correlation between processing technology and main chemical components of Dali ginseng
Objective To optimize the artillery process of Dali ginseng and to investigate the correlation between color and intrinsic quality during the artillery process.Methods A high performance liquid chromatographic(HPLC)method was established for the determination of eight ginsenosides;an ultraviolet spectrophotometric(UV)method was established for the determination of total ginsenosides and ginseng polysaccharides.Based on the Box Behnken response surface experiment,the content of ginsenoside Rg1,ginsenoside Rb1,ginsenoside Re,ginseng polysaccharide,and total ginsenosides,and the scores of the appearance properties of the decocting pieces were used as indicators,optimization of the processing technology of Ginseng preparation using AHP-entropy weighting method.Determination of colorimetric values of the powder of Dali ginseng samples with different processing technology using a colorimeter,selection of eight ginsenosides,ginseng polysaccharides and total ginsenoside content for the determination of intrinsic quality of Dali ginseng,Pearson correlation analysis,principal component analysis(PCA),hierarchical cluster analysis(HCA)and orthogonal partial least squares(OPLS-DA)were performed on the color and the content of the above indicators.Results Preferably the concoction process of Dali ginseng was 1∶10 ratio of material to liquid,the diameter of ginseng root was 2.2 cm,and the blanching time was 10 min.The results of the six validation tests showed that the average composite score of the optimal concoction process was 80.55 with a relative deviation of 1.10％from the predicted value.The results of the correlation analysis showed that ginseng polysaccharide,total ginsenosides,and ginsenosides Rg1,Rb1,Rf,Rc,Rb2,Rb3,and Rd contents showed significant positive correlations with L*,a*,b*,and E*,ginsenoside Re showed a significant positive correlation with a*,no correlation with L*,b*,E*.The results of PCA and HCA showed that the different concoctions of Dali Ginseng could be clustered into two categories:The processed samples of D1-D12 and D13-D17.OPLS-DA results show that ginsenosides Rf,Rd,and Rb1 content were important quality indicators in the process of Dali ginseng concoction,a*was an important color indicator in the process of Dali ginseng concoction.Conclusion The established Dali ginseng concoction process is stable and feasible,and the color of Dali ginseng has a certain correlation with its intrinsic quality,which can provide a reference for the subsequent evaluation of the quality of Dali ginseng.
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