Research on mechanism of Zuojin Fang targeting telocytes in anti-gastric cancer
Objective To explore the potential role of telocytes(TCs)as a target for the anti-gastric cancer effect of Zuojin Fang.Methods A mouse gastric cancer animal model was established using N-methyl-N'-nitro-N-nitrosoguanidine(MNNG)combined with H.pylori.After successful modeling,the mice were divided into model group and Zuojin Fang high,medium,and low dose(1.456,0.728,0.364 g·kg-1)groups,while the control group was not modeled.Administered ig once a day,and gave equal volumes of water to the control group and model group.Collected mouse stomach tissue specimens in week 38,selected mouse gastric tissue samples were analyzed using transmission electron microscopy(TEM),immunohistochemistry,and multiplex fluorescence immunohistochemistry to study the morphology,distribution,and immunophenotype of TCs in normal gastric tissue.Hematoxylin eosin(HE)was used to detect the pathological changes in the stomach of mice in each group,TEM observation of the morphological changes of TCs in the stomachs of mice in each group,mFIHC was used to detect the number of TCs cells in the gastric tissue of mice in each group.Results In normal mouse gastric tissue,fewer TCs were found in the mucosal layer,while TCs were relatively more abundant in the submucosal and muscular layers.The cell bodies of TCs were spindle-shaped or fusiform,with long,slender,and beaded Tps,associating with blood vessels,nerves,and smooth muscle,forming a reticular structure.The immunophenotype was CD34+/PDGFR-α+/c-kit-/CD31-,consistent with that in human gastric tissue.In mouse gastric cancer tissue,the morphology of TCs changed,the reticular structure was lost,the distribution density significantly decreased,and the number significantly reduced(P＜0.01).Compared with the model group,the Zuojin Fang groups were able to improve the changes in TCs.The high-dose group notably reduced the pathological damage to gastric tissue,and the morphology and reticular structure of TCs gradually recovered,with the number increased,showing a statistically significant difference(P＜0.01).Conclusion TCs are distributed in all layers of mouse gastric tissue,with an immunophenotype of CD34+/PDGFR-α+/c-kit-/CD31-.The reduction of TCs may be an important mechanism for the anti-gastric cancer effect of Zuo Jin Fang.
万方数据
