Bioinformatics-based analysis of oxidative stress-related genes in major depressive disorder and screening of targeted traditional Chinese medicines for prevention and treatment
Objective Bioinformatics methods were used to explore the expression patterns of oxidative stress-related differential genes in patients with major depressive disorder(MDD)and screen related traditional Chinese medicines.Methods Firstly,datasets were acquired through the GEO database and differential genes were screened using the limma package in R software.Differential oxidative stress genes were selected from the Genecards database,and common genes were obtained using a Venn diagram.Secondly,the differentially expressed genes related to oxidative stress were analyzed using Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis.Then a protein-protein interaction(PPI)network was constructed to identify Key genes for oxidative stress.Then the immune cell infiltration analysis was performed.Finally,Coremine Medical was used to predict targeted traditional Chinese medicines.Results After screening,a total of 202 differentially expressed genes were identified.KEGG enrichment analysis indicated that DEGs were mainly enriched in phagosome,Leukocyte transendothelial migration,Adipocytokine signaling pathway.Through the protein interaction(PPI)network and LASSO,the two key genes closely related to oxidative stress in MDD were identified as MOG and AIF1.The ROC curve showed that two Hub genes had high diagnostic value.The significant differential expression of these Hub genes was validated in the GSE39653dataset.Immunological pathway enrichment showed that compared to the healthy group,the NK cells resting in the MDD group were significantly increased,and the Follicular helper T cells were significantly reduced.Coremine Medical predicted that Ginseng Radix et Rhizoma,Camellia sinensis,Croci Stigma and other Chinese medicines could be used as targeted preventive or therapeutic measures for MDD.Conclusion The differential oxidative stress genes of MDD were obtained by bioinformatics analysis,which predicted that 14 preferred Chinese medicines for MDD were honey,ginseng and tea roots.
major depressive disorderoxidative stressscreening of traditional Chinese medicineGinseng Radix et RhizomaCamellia sinensisroci Stigma
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