苯酚-硫酸法测定保健酒中的粗多糖含量
张利锋 1张欣烨 1张洁 1马青青1
作者信息
- 1. 河南省疾病预防控制中心 河南郑州 450016
- 折叠
摘要
目的：：建立保健酒中粗多糖含量的测定方法。方法：采用醇提醇沉水溶法从保健酒中提取粗多糖,经苯酚-硫酸显色后用分光光度法测定其含量。结果：方法线性范围为粗多糖含量0-0.15mg/mL,回归方程为Y=0.0034X+0.0021,线性系数0.9995,加标回收率为96.2%-100.9%。结论：此方法操作简便,回收率高,可用于测定保健酒中粗多糖的含量。
Abstract
Objective:To establish a method for determination of polysaccharides in health wine.Methods:The polysaccharides in health wine which was extracted by ethanol and dissolved by hot-water, was in ultraviolet spectrophotometry determination with phenol-sulfuric acid as chromogenicagent.Results:The linear range of the method was 0-0.15mg/mL,The linear regression equation and correlation coefficient was y=0.0034x+0.0021 and 0.9995, the recoveries were 96.2%-100.9%. Conclution:This method is suitable for detecting polysaccharides in health wine with the advantages of high recovery rate and easy operation.
关键词
苯酚-硫酸法/保健酒/粗多糖Key words
phenol-sulfuric acid method/health wine/polysaccharides引用本文复制引用
出版年
2015
NETL
NSTL
维普
万方数据