Taurine is one of the necessary nutrients for animal. At Nowadays, taurine is widely used in dairy products, beverage, composite monosodium glutamate, bean products, tonic, nutrient solution, animal/aquaculture feed additive, animal, intermediates and chemical agents. It is also the delicious amino acid of fish and shrimp. Cysteine dioxygenase is one of the rate limiting enzyme in the biosynthesis of taurine. In this study, full-length of Litopenaeus vannamei cysteine dioxygenase gene (LvCDO) was obtained by race PCR. And then the characteristics of the LvCDO were analyzed. At the same time, prokaryotic expression vector was constructed to get soluble expression LvCDO, and it was purified by GST agarose. We also found that LvCDO was upregulated upon osmotic stress in haemocytes and hepatopancreas of L. vannamei.
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NSTL
维普
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