R3-MYB proteins OsTCL1 and OsTCL2 modulate seed germination via dual pathways in rice

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外文摘要：Salt and drought stress are common abiotic factors that exert a detrimental influence on seed germina-tion,potentially leading to significantly impaired growth and production in rice.Gaining a comprehen-sive understanding of the molecular response of seeds to abiotic stress during the germination is of paramount importance.In the present study,we identified two R3-MYB genes in rice,namely OsTCL1 and OsTCL2,and characterized their roles in regulating seed germination under salt and drought stress.Plants with tcl1 and tcl2 mutant alleles exhibited delayed seed germination,particularly under stress con-ditions.The tcl1 tcl2 double mutant showed an even more pronounced reduction in germination during initial stages of germination,thereby indicating a redundant regulatory function of OsTCL1 and OsTCL2 in seed germination under abiotic stresses.Furthermore,we demonstrated that the transcript levels of sev-eral phospholipase D(PLD)genes were downregulated in the tcl1 tcl2 mutant,resulting in a decreased level of the phosphatidic acid(PA)product.Application of 1-butanol,a competitive substrate inhibitor of PLD-dependent production of PA,attenuated the stress response of the tcl1 tcl2 mutant.This suggests that OsTCL1 and OsTCL2 partially modulate seed germination through the PLD-PA signaling pathway.Moreover,there were alterations in the expression of genes involved in abscisic acid(ABA)biosynthesis,metabolism and signaling transduction in the double mutant.These changes affected the endogenous ABA level and ABA response,thereby influencing seed germination.Application of both 1-butanol and ABA synthesis inhibitor sodium tungstate(Na2W04)nearly eliminated the differences in stress response between wild type and the tcl1 tcl2 mutant.This indicates that OsTCL1 and OsTCL2 synergistically coor-dinate seed germination under abiotic stresses through both the PLD-PA signaling and ABA-mediated pathways.

外文关键词：

ABADrought stressPhospholipaseSalt stressRice

作者：

Yong Yi、Chan Lin、Xueyan Peng、Meishan Zhang、Jiaming Wu、Chunmei Meng、Shengchao Ge、Yunfeng Liu、Yuan Su

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作者单位：

State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,College of Life Science and Technology,Guangxi University,Nanning 530004,Guangxi,China

Life Sciences Institute,Guangxi Medical University,Nanning 530021,Guangxi,China

基金：

National Natural Science Foundation of ChinaNatural Science Foundation of Guangxi provinceState Key Laboratory for Conservation and Utilization of Subtropical AgrobioresourcesState Key Laboratory for Conservation and Utilization of Subtropical AgrobioresourcesGuangxi University-Bama Research Fund

项目编号：

319706032019GXNSFDA185001SKLCUSA-a202008SKLCUSA-a0120220006

出版年：

2023

DOI：

10.1016/j.cj.2023.10.004

作物学报(英文版)

CSTPCDCSCD

ISSN：

年,卷(期)：2023.11(6)

参考文献量51