Genome-wide association with transcriptomics reveals a shade-tolerance gene network in soybean

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外文摘要：Shade tolerance is essential for soybeans in inter/relay cropping systems.A genome-wide association study(GWAS)integrated with transcriptome sequencing was performed to identify genes and construct a genetic network governing the trait in a set of recombinant inbred lines derived from two soybean par-ents with contrasting shade tolerance.An improved GWAS procedure,restricted two-stage multi-locus genome-wide association study based on gene/allele sequence markers(GASM-RTM-GWAS),identified 140 genes and their alleles associated with shade-tolerance index(STI),146 with relative pith cell length(RCL),and nine with both.Annotation of these genes by biological categories allowed the construction of a protein-protein interaction network by 187 genes,of which half were differentially expressed under shading and non-shading conditions as well as at different growth stages.From the identified genes,three ones jointly identified for both traits by both GWAS and transcriptome and two genes with maxi-mum links were chosen as beginners for entrance into the network.Altogether,both STI and RCL gene systems worked for shade-tolerance with genes interacted each other,this confirmed that shade-tolerance is regulated by more than single group of interacted genes,involving multiple biological func-tions as a gene network.

外文关键词：

Soybean(Glycine max(L.)Merr.)Shade-toleranceRestricted two-stage multi-locus genome-wide association study based on gene/allele sequence markers(GASM-RTM-GWAS)Shade-tolerance index(STI)Relative cell length(RCL)Transcriptome

作者：

Yanzhu Su、Xiaoshuai Hao、Weiying Zeng、Zhenguang Lai、Yongpeng Pan、Can Wang、Pengfei Guo、Zhipeng Zhang、Jianbo He、Guangnan Xing、Wubin Wang、Jiaoping Zhang、Zudong Sun、Junyi Gai

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作者单位：

Soybean Research Institute/MARA National Center for Soybean Improvement MARA Key Laboratory of Biology and Genetic Improvement of Soybean/State Key Laboratory for Crop Genetics and Germplasm Enhancement/State Innovation Platform for Integrated Production and Education in Soybean Bio-breeding/Jiangsu Collaborative Innovation Center for Modern Crop Production,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China

Guangxi Academy of Agricultural Sciences,Nanning 530007,Guangxi,China

基金：

National Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaMOE 111 ProjectMO A CARS-04 programProgram of Jiangsu provinceGuangxi Scientific Research and Technology Development PlanGuidance Foundation of Sanya Institute of Nanjing Agricultural UniversityGuidance Foundation of Sanya Institute of Nanjing Agricultural University

项目编号：

2021YFF10012042021YFD1201602B08025JBGS-2021-01414125008-2-16NAUSY-ZZ02NAUSY-MS05

出版年：

2024

DOI：

10.1016/j.cj.2023.11.013

作物学报(英文版)

CSTPCD

ISSN：

年,卷(期)：2024.12(1)

参考文献量59