首页|Identification and fine mapping of qSW2 for leaf slow wilting in soybean

Identification and fine mapping of qSW2 for leaf slow wilting in soybean

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Drought is one of the abiotic stresses limiting the production of soybean(Glycine max).Elucidation of the genetic and molecular basis of the slow-wilting(SW)trait of this crop offers the prospect of its genetic improvement.A panel of 188 accessions and a set of recombinant inbred lines produced from a cross between cultivars Liaodou 14 and Liaodou 21 were used to identify quantitative-trait loci(QTL)associ-ated with SW.Plants were genotyped by Specific-locus amplified fragment sequencing and seedling leaf wilting was assessed under three water-stress treatments.A genome-wide association study identified 26 SW-associated single-nucleotide polymorphisms(SNPs),including three located in a 248-kb linkage-disequilibrium(LD)block on chromosome 2.Linkage mapping revealed a major-effect QTL,qSW2,associated with all three treatments and adjacent to the LD block.Fine mapping in a BC2F3 popu-lation derived from a backcross between Liaodou 21 and R26 confined qSW2 to a 60-kb interval.Gene expression and sequence variation analysis identified the gene Glyma.02 g218100,encoding an auxin transcription factor,as a candidate gene for qSW2.Our results will contribute significantly to improving drought-resistant soybean cultivars by providing genetic information and resources.

DroughtGWASLinkage mappingSlow wiltingSoybean(Glycine max)

Shengyou Li、Changling Wang、Chunjuan Yan、Xugang Sun、Lijun Zhang、Yongqiang Cao、Wenbin Wang、Shuhong Song

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Institute of Crop Research,Liaoning Academy of Agricultural Sciences,Shenyang 110161,Liaoning,China

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Key Research and Development Program of ChinaLiaoning Provincial Major Special Project of Agricultural Science and TechnologyLiaoning Provincial Major Special Project of Agricultural Science and TechnologyKey Research and Development Plan of Liaoning Science and Technology DepartmentShenyang Seed Industry Innovation Project

32101795323017822016YFD0100201-012022JH1/102000022021JH1/104000382021JH2/102002722-318-2-12

2024

10.1016/j.cj.2023.10.013

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(1)
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