首页|Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L.

Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L.

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Sesame Fusarium wilt(SFW),caused by Fusarium oxysporum f.sp.sesami(Fos),is one of the most devas-tating diseases affecting sesame cultivation.Deciphering the genetic control of SFW resistance is pivotal for effective disease management in sesame.An inheritance study on a cross between the highly resistant variety Yuzhi 11 and the highly susceptible accession Sp1 using a Fos pathogenicity group 1 isolate indi-cated that resistance was conferred by a single dominant allele.The target locus was located in a 1.24 Mb interval on chromosome 3 using a combination of cross-population association mapping and bulked seg-regant analysis.Fine genetic mapping further narrowed the interval between 21,350 and 21,401 kb.The locus Sindi_0812400 was identified as the SFW resistance gene and officially designated SiRLK1.This gene encodes a specific malectin/receptor-like protein kinase with three putative tandem kinase domains and is considered a kinase fusion protein.Sequence analysis revealed that a high proportion(49.44％)of vari-ants within the locus was located within the kinase domain Ⅲ,and several of which were evidently asso-ciated with the diversity in SFW response,indicating the critical role of kinase domain Ⅲ in expression of disease resistance.These findings provide valuable information for further functional analysis of SFW resistance genes and marker-assisted resistance breeding in sesame.

Breeding for resistanceGenetic analysisMolecular analysisTandem kinase domain

Yinghui Duan、Wenwen Qu、Shuxian Chang、Ming Ju、Cuiying Wang、Cong Mu、Hengchun Cao、Guiting Li、Qiuzhen Tian、Qin Ma、Zhanyou Zhang、Haiyang Zhang、Hongmei Miao

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The Shennong Laboratory,Zhengzhou 450002,Henan,China

Henan Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China

Key Laboratory of Specific Oilseed Crops Genomics of Henan Province,Henan Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China

China Agriculture Research SystemKey Research and Development Project of Henan ProvinceHenan Provincial Science and Technology Research ProjectZhongyuan Science and Technology Innovation Leading Talent PlanKey Research Project of the Shennong LaboratoryFund for Distinguished Young Scholars from Henan Academy of Agricultural Sciences

CARS-14221111520400222102110081214200510020SN01-2022-042022JQ01

2024

10.1016/j.cj.2023.12.004

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(1)
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