首页|Elite,transformable haploid inducers in maize

Elite,transformable haploid inducers in maize

扫码查看
原文链接
  • 万方数据
  • 维普
The introduction of alleles into commercial crop breeding pipelines is both time consuming and costly.Two technologies that are disrupting traditional breeding processes are doubled haploid(DH)breeding and genome editing(GE).Recently,these techniques were combined into a GE trait delivery system called HI-Edit(Haploid Inducer-Edit).In HI-Edit,the pollen of a haploid inducer line is reprogrammed to deliver GE traits to any variety,obviating recurrent selection.For HI-Edit to operate at scale,an efficient transformable HI line is needed,but most maize varieties are recalcitrant to transformation,and haploid inducers are especially difficult to transform given their aberrant reproductive behaviors.Leveraging marker assisted selection and a three-tiered testing scheme,we report the development of new Iodent and Stiff Stalk maize germplasm that are transformable,have high haploid induction rates,and exhibit a robust,genetically-dominant anthocyanin native trait that may be used for rapid haploid identification.We show that transformation of these elite"HI-Edit"lines is enhanced using the BABYBOOM and WUSCHEL morphogenetic factors.Finally,we evaluate the HI-Edit performance of one of the lines against both Stiff Stalk and non-Stiff Stalk testers.The strategy and results of this study should facilitate the development of commercially scalable HI-Edit systems in diverse crops.

Zea mays L.Doubled haploidsTransformationGenome editingQTL

Brent Delzer、Dawei Liang、David Szwerdszarf、Isadora Rodriguez、Gonzalo Mardones、Sivamani Elumalai、Francine Johnson、Samson Nalapalli、Rachel Egger、Erin Burch、Kerry Meier、Juan Wei、Xiujuan Zhang、Huaping Gui、Huaibing Jin、Huan Guo、Kun Yu、Yubo Liu、Becky Breitinger、Ana Poets、Jason Nichols、Wan Shi、David Skibbe、Qiudeng Que、Timothy Kelliher

展开 >

Seeds Development,Syngenta Seeds,Janesville,WI 53546,USA

Seeds Research,Syngenta Biotechnology China Co.,Ltd.,Beijing 102206,China

Centro de Investigación de Cultivos Arica,Syngenta R&D,Arica 1000000,Chile

Syngenta S.A.,Proyecto la Soledad,Graneros 2880000,Chile

Seeds Research,Syngenta Crop Protection LLC,RTP,NC 27709,USA

Seeds Development,Syngenta Seeds,Slater,IA 50244,USA

展开 >

2024

10.1016/j.cj.2023.10.016

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(1)
  • 29