首页|Enemies at peace:Recent progress in Agrobacterium-mediated cereal transformation

Enemies at peace:Recent progress in Agrobacterium-mediated cereal transformation

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Agrobacterium tumefaciens mediated plant transformation is a versatile tool for plant genetic engineering following its discovery nearly half a century ago.Numerous modifications were made in its application to increase efficiency,especially in the recalcitrant major cereals plants.Recent breakthroughs in transfor-mation efficiency continue its role as a mainstream technique in CRISPR/Cas-based genome editing and gene stacking.These modifications led to higher transformation frequency and lower but more stable transgene copies with the capability to revolutionize modern agriculture.In this review,we provide a brief overview of the history of Agrobacterium-mediated plant transformation and focus on the most recent progress to improve the system in both the Agrobacterium and the host recipient.A promising future for transformation in biotechnology and agriculture is predicted.

Agrobacterium tumefaciensCereal speciesGenome editingGenetic engineeringPlant breeding

Shaoshuai Liu、Ke Wang、Shuaifeng Geng、Moammar Hossain、Xingguo Ye、Aili Li、Long Mao、Karl-Heinz Kogel

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State Key Laboratory of Crop Gene Resources and Breeding,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

Institute of Phytopathology,Centre for BioSystems,Land Use and Nutrition,Justus Liebig University,Heinrich Buff-Ring 26-32,D-35392,Giessen,Germany

High-End Foreign Expert Recruitment Program国家自然科学基金Hainan Yazhou Bay Seed LabAgricul-tural Science and Technology Innovation Program of CAASAgricul-tural Science and Technology Innovation Program of CAAS河北省自然科学基金"Yellow River Delta Scholar"in Sino-Agro Experimental Station for Salt Tolerant Crops(SAESSTC),Dongying,Shandong,ChinaGerman Minister for Bildung und Forschung(BMBF)in the project Wheat InterferePostdoc Practice Station of Shandong ProvinceRay Dixon Academician Station at SAESSTC

G2022051003L32201878B21HJ0215CAAS-ZDRW202002CAAS-ZDRW202201C2021205013

2024

10.1016/j.cj.2023.12.009

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(2)
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