The cytosolic isoform of triosephosphate isomerase,ZmTPI4,is required for kernel development and starch synthesis in maize(Zea mays L.)

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外文摘要：Triosephosphate isomerase(TPI)is an enzyme that functions in plant energy production,accumulation,and conversion.To understand its function in maize,we characterized a maize TPI mutant,zmtpi4.In comparison to the wild type,zmtpi4 mutants showed altered ear development,reduced kernel weight and starch content,modified starch granule morphology,and altered amylose and amylopectin content.Protein,ATP,and pyruvate contents were reduced,indicating ZmTPI4 was involved in glycolysis.Although subcellular localization confirmed ZmTPI4 as a cytosolic rather than a plastid isoform of TPI,the zmtpi4 mutant showed reduced leaf size and chlorophyll content.Overexpression of ZmTPI4 in Arabidopsis led to enlarged leaves and increased seed weight,suggesting a positive regulatory role of ZmTPI4 in kernel weight and starch content.We conclude that ZmTPI4 functions in maize kernel devel-opment,starch synthesis,glycolysis,and photosynthesis.

外文关键词：

MaizeKernelStarchWeightPhotosynthesis

作者：

Wenyu Li、Han Wang、Qiuyue Xu、Long Zhang、Yan Wang、Yongbiao Yu、Xiangkun Guo、Zhiwei Zhang、Yongbin Dong、Yuling Li

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作者单位：

State Key Laboratory of Wheat and Maize Crop Science,Collaborative Innovation Center of Henan Grain Crops,College of Agronomy,Henan Agricultural University,Longzi Lake Campus,Zhengzhou 450046,Henan,China

基金：

Major Public Welfare Projects of Henan ProvinceZhongyuan Schol-ars in Henan ProvinceTackle Program of Agricultural Seed in Henan ProvinceTechnical System of Maize Industry in Henan ProvinceState Key Laboratory of Wheat and Maize Crop Science

项目编号：

201300111100224005100032022010201HARS-22-02-SSKL2023ZZ05

出版年：

2024

DOI：

10.1016/j.cj.2024.02.001

作物学报(英文版)

CSTPCD

ISSN：

年,卷(期)：2024.12(2)

参考文献量30