首页|Suppressing a mitochondrial calcium uniporter activates the calcium signaling pathway and promotes cell elongation in cotton
Suppressing a mitochondrial calcium uniporter activates the calcium signaling pathway and promotes cell elongation in cotton
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Mitochondrial calcium uniporter(MCU)is a conserved calcium ion(Ca2+)transporter in the mitochon-drial inner membrane of eukaryotic cells.How MCU proteins regulate Ca2+flow and modulate plant cell development remain largely unclear.Here,we identified the gene GhMCU4 encoding a MCU protein that negatively regulates plant development and fiber elongation in cotton(Gossypium hirsutum).GhMCU4 expressed constitutively in various tissues with the higher transcripts in elongating fiber cells.Knockdown of GhMCU4 in cotton significantly elevated the plant height and root length.The calcium sig-naling pathway was significantly activated and calcium sensor genes,including Ca2+dependent modula-tor of interactor of constitutively active ROP(GhCMI1),calmodulin like protein(GhCML46),calcium-dependent protein kinases(GhCPKs),calcineurin B-like protein(GhCBLs),and CBL-interacting protein kinases(GhCIPKs),were dramatically upregulated in GhMCU4-silenced plants.Metabolic processes were preferentially enriched,and genes related to regulation of transcription were upregulated in GhMCU4-si-lenced plants.The contents of Ca2+and H2O2 were significantly increased in roots and leaves of GhMCU4-silenced plants.Fiber length and Ca2+and H2O2 contents in fibers were significantly increased in GhMCU4-silenced plants.This study indicated that GhMCU4 plays a negative role in regulating cell elon-gation in cotton,thus expanding understanding in the role of MCU proteins in plant growth and development.
State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China
Engineering Research Center of Ministry of Education for Cotton Germplasm Enhancement and Application,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China
National Key Research and Devel-opment Program of ChinaJiangsu Key R & D ProgramCollaborative Innovation Center for Modern Crop Production co-sponsored by Province and Minis-try(CIC-MCP)
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