首页|SsdchA is a novel secretory cellobiohydrolase driving pathogenicity in Sclerotinia sclerotiorum
SsdchA is a novel secretory cellobiohydrolase driving pathogenicity in Sclerotinia sclerotiorum
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The necrotrophic fungus,Sclerotinia sclerotiorum,employs an array of cell wall-degrading enzymes(CWDEs),including cellulase,to dismantle host cell walls.However,the molecular mechanisms through which S.sclerotiorum degrades cellulose remain elusive.Here,we unveil a novel secretory cellobiohydro-lase,SsdchA,characterized by a signal peptide and a Glyco_hydro_7(GH7)domain.SsdchA exhibits a robust expression of during early infection stages.Interestingly,colony morphology and growth rates remain unaffected across the wild-type,SsdchA deletion strains and SsdchA overexpression strains on potato dextrose agar(PDA)medium.Nevertheless,the pathogenicity and cellobiohydrolase activity decreased in the SsdchA deletion strains,but enhanced in the SsdchA overexpression strains.Moreover,the heterologous expression of SsdchA in Arabidopsis thaliana leads to reduced cellulose content and heightened susceptibility to S.sclerotiorum.Collectively,our data underscore the pivotal role of the novel cellobiohydrolase SsdchA in the pathogenicity of S.sclerotiorum.
Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City,College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China
Academy of Agricultural Sciences,Southwest University,Chongqing 400715,China
Engineering Research Center of South Upland Agriculture,Ministry of Education,Chongqing 400715,China
College of Plant Protection,Southwest University,Chongqing 400715,China
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国家自然科学基金Project of Chongqing Science and Technology Commission中央高校基本科研业务费专项
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