首页|Knock-out of BnHva22c reduces the susceptibility of Brassica napus to infection with the fungal pathogen Verticillium longisporum

Knock-out of BnHva22c reduces the susceptibility of Brassica napus to infection with the fungal pathogen Verticillium longisporum

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Verticillium longisporum(Vl43)is a soilborne hemibiotrophic fungal pathogen causing stem striping on oilseed rape(OSR)and severe yield losses.Breeding for resistant varieties is the most promising approach to control this disease.Here,we report the identification of Hva22c as a novel susceptibility factor and its potential for improving OSR resistance.Hva22c is a member of the Hva22 gene family,originally described for barley(Hordeum vulgare).Several Hva22 members have been located at the endoplasmic reticulum.Hva22c is up-regulated in response to Vl43 in both Arabidopsis and OSR.We demonstrate that knock-out of Hva22c in OSR by CRISPR/Cas9 and its homolog in Arabidopsis by T-DNA insertion reduced plants'susceptibility to Vl43 infection and impaired the development of disease symptoms.To under-stand the underlying mechanism,we analysed transcriptomic data from infected and non-infected roots of hva22c knock-out and wild type plants.We identified a homozygous mutant with frame-shifts in all four BnHva22c loci displaying a vastly altered transcriptional landscape at 6 dpi.Significantly,a large set of genes was suppressed under mock conditions including genes related to the endomembrane sys-tems.Among the up-regulated genes we found several defense-related and phytohormone-responsive genes when comparing mutant to the wild type.These results demonstrate that Hva22c is functionally required for a fully compatible plant-fungus interaction.Its loss of function reduces plant susceptibility,most likely due to endoplasmatic reticulum and Golgi dysfunction accompanied by additionally activated defense responses.These findings can help improve OSR resistance to V.longisporum infection.

Hva22cSusceptibility factorCRISPR/Cas9Brassica napusArabidopsisVerticillium longisporumResistance breeding

Wanzhi Ye、Roxana Hossain、Michael Pr?bsting、Abdallah Abdelmegid Mohamed Ali、Lingyue Han、Ying Miao、Steffen Rietz、Daguang Cai、Dirk Schenke

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Department of Molecular Phytopathology and Biotechnology,Institute of Phytopathology,Christian-Albrechts-University of Kiel,Kiel,Germany

Institut für Zuckerrübenforschung,Göttingen,Germany

Plant Pathology Department,Faculty of Agriculture and Natural Resources,Aswan University,Aswan,Egypt

Fujian Provincial Key Laboratory of Plant Functional Biology,College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China

Hohenlieth-Hof,NPZ Innovation GmbH,Holtsee,Germany

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德国联邦教研部项目Bundesministerium für Ern?hrung und LandwirtschaftBunde-sanstalt für Landwirtschaft and Ern?hrung

031B0033C220065162814IP004

2024

10.1016/j.cj.2024.02.012

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(2)
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