首页|Mapping of powdery mildew resistance genes transferred to common wheat from wild emmer wheat revealed three functional Pm60 haplotypes

Mapping of powdery mildew resistance genes transferred to common wheat from wild emmer wheat revealed three functional Pm60 haplotypes

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Powdery mildew(PM),caused by Blumeria graminis f.sp.tritici(Bgt),is one of the destructive wheat dis-eases worldwide.Wild emmer wheat(Triticum turgidum ssp.dicoccoides,WEW),a tetraploid progenitor of common wheat,is a valuable genetic resource for wheat disease resistance breeding programs.We developed three hexaploid pre-breeding lines with PM resistance genes derived from three WEW acces-sions.These resistant pre-breeding lines were crossed with susceptible common wheat accessions.Segregations in the F2 populations were 3 resistant:1 susceptible,suggesting a single dominant allele in each resistant parent.Mapping of the resistance gene in each line indicated a single locus on the long arm of chromosome 7A,at the approximate location of previously cloned Pm60 from T.urartu.Sanger sequencing revealed three different Pm60 haplotypes(Hap 3,Hap 5,and Hap 6).Co-segregating diagnos-tic markers were developed for identification and selection of each haplotype.The resistance function of each haplotype was verified by the virus-induced gene silencing(VIGS).Common wheat lines carrying each of these Pm60 haplotypes were resistant to most Bgt isolates and differences in the response arrays suggested allelic variation in response.

AllelesBlumeria graminusMarker-assisted selectionMolecular markerTriticum dicoccoides

Wenxin Wei、Nannan Liu、Shengnan Zhang、Jing Zhang、Wei Pan、Xiaoming Xie、Zuhuan Yang、Junna Sun、Jun Ma、Zhaorong Hu、Weilong Guo、Qiaoling Luo、Jingzhong Xie、Fei He、Yinghui Li、Chaojie Xie、Qixin Sun

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Key Laboratory of Crop Heterosis and Utilization(MOE)/Key Laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100193,China

Institute of Advanced Agricultural Science,Peking University,Weifang 261200,Shandong,China

Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China

Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China

Institute of Evolution,University of Haifa,Mt.Carmel,Haifa 3498838,Israel

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国家重点研发计划国家重点研发计划国家自然科学基金

2023YFF10004042022YFF1000150132171971

2024

10.1016/j.cj.2024.01.015

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(2)
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