Multi-environment BSA-seq using large F3 populations is able to achieve reliable QTL mapping with high power and resolution:An experimental demonstration in rice

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外文摘要：Bulked-segregant analysis by deep sequencing(BSA-seq)is a widely used method for mapping QTL(quantitative trait loci)due to its simplicity,speed,cost-effectiveness,and efficiency.However,the ability of BSA-seq to detect QTL is often limited by inappropriate experimental designs,as evidenced by numer-ous practical studies.Most BSA-seq studies have utilized small to medium-sized populations,with F2 populations being the most common choice.Nevertheless,theoretical studies have shown that using a large population with an appropriate pool size can significantly enhance the power and resolution of QTL detection in BSA-seq,with F3 populations offering notable advantages over F2 populations.To pro-vide an experimental demonstration,we tested the power of BSA-seq to identify QTL controlling days from sowing to heading(DTH)in a 7200-plant rice F3 population in two environments,with a pool size of approximately 500.Each experiment identified 34 QTL,an order of magnitude greater than reported in most BSA-seq experiments,of which 23 were detected in both experiments,with 17 of these located near 41 previously reported QTL and eight cloned genes known to control DTH in rice.These results indicate that QTL mapping by BSA-seq in large F3 populations and multi-environment experiments can achieve high power,resolution,and reliability.

外文关键词：

BSA-seqQTL mappingLarge F3 populationMulti-environment experimentCross-validation

作者：

Yan Zheng、Ei Ei Khine、Khin Mar Thi、Ei Ei Nyein、Likun Huang、Lihui Lin、Xiaofang Xie、Min Htay Wai Lin、Khin Than Oo、Myat Myat Moe、San San Aye、Weiren Wu

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作者单位：

College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China

Fujian Provincial Key Laboratory of Crop Breeding by Design,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China

Key Laboratory of Genetics,Breeding and Multiple Utilization of Crops,Ministry of Education,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China

Department of Botany,Mawlamyine University,Mon State,Mawlamyine,Myanmar

Department of Botany,Dagon University,North Dagon,Yangon,Myanmar

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基金：

福建省自然科学基金福建省自然科学基金Cooperation Project on University Industry-Education-Research of Fujian Provincial Science and Technology Plan(CN)Lancang-Mekong Cooperation Special Fund(2017-2020)International Sci-Tech Cooperation and Communication Program of Fujian Agriculture and Forestry University

项目编号：

2020I00092022J015962022N5011KXGH17014

出版年：

2024

DOI：

10.1016/j.cj.2024.01.009

作物学报(英文版)

CSTPCD

ISSN：

年,卷(期)：2024.12(2)

参考文献量36