首页|A novel semi-dominant allele of the transmembrane NAC transcription factor ZmNTL2 reduces the size of multiple maize organs

A novel semi-dominant allele of the transmembrane NAC transcription factor ZmNTL2 reduces the size of multiple maize organs

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Maize plant architecture influences planting density and,in turn,grain yield.Most of the plant architecture-related traits can be described as organ size.We describe a miniature maize mutant,Tiny plant 4(Tip4),which exhibits reduced size of multiple organs and exhibits a semi-dominant monofacto-rial inheritance characteristic.Positional cloning confirmed that a 4-bp deletion in the NAC TF with trans-membrane motif 1-Like(NTL)gene ZmNTL2,denoted as ZmNTL2△,confers the Tip4 mutation.qRT-PCR showed that ZmNTL2 was expressed in all tested tissues.ZmNTL2 functions as a transcriptional activator and is located in both the nucleus and biomembranes.The mutation does not affect the mRNA abundance of ZmNTL2 locus,but it does result in the loss of transmembrane domain and confines the ZmNTL2△ pro-tein to the nucleus.Knocking out ZmNTL2 has no effect on maize organ size development,indicating that the 4-bp deletion might be a gain-of-function mutation in organ size regulation.Combining transcrip-tome sequencing with cytokinin and auxin content determination suggests that the decreased organ size may be possibly mediated by changes in hormone homeostasis.

Organ sizeγ-ray mutagenesisMembrane-bound NAC TFHormone homeostasisMaize

Chuan Li、Yun Bai、Yuwei Hou、Siyu Wang、Yue Xin、Tao Yu、Jing Wang、Xiaowei Liu、Hongyang Yi、Chao Xia、Hai Lan、Moju Cao

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State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Maize Research Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China

Jilin Engineering Research Center for Crop Biotechnology Breeding,College of Plant Science,Jilin University,Changchun 130012,Jilin,China

National Key Research and Development Program of ChinaCrop Varietal Improvement and Insect Pests Control by Nuclear Radiation,Platform for Mutation Breeding by Radiation of SichuanNatural Science Foundation of Sichuan ProvinceApplied Basic Research Programs of Sichuan Provincial Science and Technology Department

2022YFD1200704--32021YFYZ00112022NSFSC16352020YJ0249

2024

10.1016/j.cj.2024.04.002

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(3)
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