Wild soybean(Glycine soja)transcription factor GsWRKY40 plays positive roles in plant salt tolerance

扫码查看

原文链接

NETL
NSTL
万方数据

外文摘要：Wild soybean(Glycine soja),a relative of cultivated soybean,shows high adaptability to adverse environ-mental conditions.We identified and characterized a wild soybean transcription factor gene,GsWRKY40,that promotes plant salt stress.GsWRKY40 was highly expressed in wild soybean roots and was up-regulated by salt treatment.GsWRKY40 was localized in nucleus and demonstrated DNA-binding activ-ities but without transcriptional activation.Mutation and overexpression of GsWRKY40 altered salt toler-ance of Arabidopsis plants.To understand the molecular mechanism of GsWRKY40 in regulating plant salt resistance,we screened a cDNA library and identified a GsWRKY40 interacting protein GsbHLH92 by using yeast two-hybrid approach.The physical interaction of GsWRKY40 and GsbHLH92 was confirmed by co-immunoprecipitation(co-IP),GST pull-down,and bimolecular fluorescence complementation(BiFC)techniques.Intriguingly,co-overexpression of GsWRKY40 and GsbHLH92 resulted in higher salt tol-erance and lower ROS levels than overexpression of GsWRKY40 or GsbHLH92 in composite soybean plants,suggesting that GsWRKY40 and GsbHLH92 may synergistically regulate plant salt resistance through inhibiting ROS production.qRT-PCR data indicated that the expression level of GmSPOD1 gene encoding peroxidase was cooperatively regulated by GsWRKY40 and GsbHLH92,which was confirmed by using a dual luciferase report system and yeast one-hybrid experiment.Our study reveals a pathway that GsWRKY40 and GsbHLH92 collaboratively up-regulate plant salt resistance through impeding GmSPOD1 expression and reducing ROS levels,providing a novel perspective on the regulatory mecha-nisms underlying plant tolerance to abiotic stresses.

外文关键词：

Wild soybeanTranscription factorSalt stressROS

作者：

Minglong Li、Man Xue、Huiying Ma、Peng Feng、Tong Chen、Xiaohuan Sun、Qiang Li、Xiaodong Ding、Shuzhen Zhang、Jialei Xiao

展开 >

作者单位：

Key Laboratory of Agricultural Biological Functional Genes,Northeast Agricultural University,Harbin 150030,Heilongjiang,China

Key Laboratory of Soybean Biology of Chinese Education Ministry,Northeast Agricultural University,Harbin 150030,Heilongjiang,China

College of Life Science,Leshan Normal University,Leshan 614000,Sichuan,China

Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences,Mudanjiang 157401,Heilongjiang,China

Department of Plant Science,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China

展开 >

基金：

National Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNatural Science Foundation of Heilongjiang Province

项目编号：

2021YFD1201104-023227204832272017LH2022C019

出版年：

2024

DOI：

10.1016/j.cj.2024.03.011

作物学报(英文版)

CSTPCD

ISSN：

年,卷(期)：2024.12(3)