首页|Fine mapping and characterization of stripe rust resistance gene YrAYH in near-isogenic lines derived from a cross involving wheat landrace Anyuehong

Fine mapping and characterization of stripe rust resistance gene YrAYH in near-isogenic lines derived from a cross involving wheat landrace Anyuehong

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Stripe rust,caused by Puccinia striiformis f.sp.tritici(Pst),is a devastating disease in wheat worldwide.Discovering and characterizing new resistance genes/QTL is crucial for wheat breeding programs.In this study,we fine-mapped and characterized a stripe rust resistance gene,YRAYH,on chromosome arm 5BL in the Chinese wheat landrace Anyuehong(AYH).Evaluations of stripe rust response to prevalent Chinese Pst races in near-isogenic lines derived from a cross of Anyuehong and Taichung 29 showed that YrAYH conferred a high level of resistance at all growth stages.Fine mapping using a large segregating popula-tion of 9748 plants,narrowed the YRAYH locus to a 3.7 Mb interval on chromosome arm 5BL that included 61 annotated genes.Transcriptome analysis of two NIL pairs identified 64 upregulated differentially expressed genes(DEGs)in the resistant NILs(NILs-R).Annotations indicated that many of these genes have roles in plant disease resistance pathways.Through a combined approach of fine-mapping and tran-scriptome sequencing,we identified a serine/threonine-protein kinase SRPK as a candidate gene under-lying YrAYH.A unique 25 bp insertion was identified in the NILs-R compared to the NILs-S and previously published wheat genomes.An InDel marker was developed and co-segregated with YrAYH.Agronomic trait evaluation of the NILs suggested that YrAYH not only reduces the impact of stripe rust but was also associated with a gene that increases plant height and spike length.

Candidate gene analysisCrop protectionPuccinia striiformisTranscriptome analyses

Li Long、Jue Li、Linyu Huang、Huiling Jin、Fangnian Guan、Haipeng Zhang、Sasa Zhao、Hao Li、Zhien Pu、Wei Li、Qiantao Jiang、Yuming Wei、Jian Ma、Houyang Kang、Shoufen Dai、Pengfei Qi、Qiang Xu、Mei Deng、Youliang Zheng、Yunfeng Jiang、Matthew James Moscou、Guoyue Chen

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State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Sichuan Agricultural University,Chengdu 611130,Sichuan,China

Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China

The Sainsbury Laboratory,Norwich Research Park,Norwich NR4 7UH,England,UK

College of Resources,Sichuan Agricultural University,Chengdu 611130,Sichuan,China

United States Department of Agriculture-Agricultural Research Service,Cereal Disease Laboratory,St.Paul,MN 55108,USA

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Major Program of National Agricultural Science and Technology of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaScience and Technology Department of Sichuan ProvinceScience and Technology Department of Sichuan ProvinceScience and Technology Department of Sichuan Province

NK2022060732272059319718832021YFYZ00022022ZDZX00142023NSFSC1995

2024

10.1016/j.cj.2024.03.009

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(3)
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