首页|GmAP1d regulates flowering time under long-day photoperiods in soybean

GmAP1d regulates flowering time under long-day photoperiods in soybean

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Flowering time is important for adaptation of soybean(Glycine max)to different environments.Here,we conducted a genome-wide association study of flowering time using a panel of 1490 cultivated soybean accessions.We identified three strong signals at the qFT02-2 locus(Chr02:12037319-12238569),which were associated with flowering time in three environments:Gongzhuling,Mengcheng,and Nanchang.By analyzing linkage disequilibrium,gene expression patterns,gene annotation,and the diversity of vari-ants,we identified an API homolog as the candidate gene for the qFT02-2 locus,which we named GmAP1d.Only one nonsynonymous polymorphism existed among 1490 soybean accessions at position Chr02:12087053.Accessions carrying the Chr02:12087053-T allele flowered significantly earlier than those carrying the Chr02:12087053-A allele.Thus,we developed a cleaved amplified polymorphic sequence(CAPS)marker for the SNP at Chr02:12087053,which is suitable for marker-assisted breeding of flowering time.Knockout of GmAP1d in the'Williams 82'background by gene editing promoted flow-ering under long-day conditions,confirming that GmAP1d is the causal gene for qFT02-2.An analysis of the region surrounding GmAP1d revealed that GmAP1d was artificially selected during the genetic improvement of soybean.Through stepwise selection,the proportion of modern cultivars carrying the Chr02:12087053-T allele has increased,and this allele has become nearly fixed(95％)in northern China.These findings provide a theoretical basis for better understanding the molecular regulatory mech-anism of flowering time in soybean and a target gene that can be used for breeding modern soybean cul-tivars adapted to different latitudes.

SoybeanFlowering timeGWASGmAP1dLong-day conditions

Shiyu Guo、Yanfei Li、Hongmei Qiu、Guoyu Hu、Chaosen Zhao、Ruizhen Wang、Hao Zhang、Yu Tian、Xiaoyu Li、Bin Liu、Ying-hui Li、Li-juan Qiu

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College of Agriculture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China

State Key Laboratory of Crop Gene Resources and Breeding/The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization(MARA),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

Key Lab of Chinese Medicine Resources Conservation,State Administration of Traditional Chinese Medicine of the People's Republic of China,Institute of Medicinal Plant Development,Chinese Academy of Medical

Jilin Academy of Agricultural Sciences/National Engineering Research Center for Soybean,Changchun 130033,Jilin,China

Crop Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,Anhui,China

Crops Research Institute of Jiangxi Academy of Agricultural Sciences/Jiangxi Province Key Laboratory of Oilcrops Biology,Nanchang 330200,Jiangxi,China

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National Natural Science Foundation of ChinaNational Key Research and Development Program of ChinaChina Agriculture Research SystemAgricultural Science and Technology Innovation Program(ASTIP)of Chinese Academy of Agricultural Sciences,Scientific InnovatiPlatform of National Crop Germplasm Resources of China

U22A204732021YFD1201600CARS-04-PS012022ZD0401703

2024

10.1016/j.cj.2024.03.004

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(3)
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