Candidate genes conferring ethylene-response in cultivated peanuts determined by BSA-seq and fine-mapping

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外文摘要：Ethylene plays essential roles in plant growth,development and stress responses.The ethylene signaling pathway and molecular mechanism have been studied extensively in Arabidopsis and rice but limited in peanuts.Here,we established a sand-culture method to screen pingyangmycin mutagenized peanut lines based on their specific response to ethylene("triple response").An ethylene-insensitive mutant,inhibi-tion of peanut hypocotyl elongation 1(iph1),was identified that showed reduced sensitivity to ethylene in both hypocotyl elongation and root growth.Through bulked segregant analysis sequencing,a major gene related to iph1,named AhIPH1,was preliminarily mapped at the chromosome Arahy.01,and further narrowed to a 450-kb genomic region through substitution mapping strategy.A total of 7014 genes were differentially expressed among the ACC treatment through RNA-seq analysis,of which only the Arahy.5BLU0Q gene in the candidate mapping interval was differentially expressed between WT and mutant iph1.Integrating sequence variations,functional annotation and transcriptome analysis revealed that a predicated gene,Arahy.5BLU0Q,encoding SNF1 protein kinase,may be the candidate gene for AhIPH1.This gene contained two single-nucleotide polymorphisms at promoter region and was more highly expressed in iph1 than WT.Our findings reveal a novel ethylene-responsive gene,which provides a theoretical foundation and new genetic resources for the mechanism of ethylene signaling in peanuts.

外文关键词：

Ethylene-insensitiveHypocotyl elongationAhIPH1Candidate geneGenetic resources

作者：

Yanyan Tang、Zhong Huang、Shaohui Xu、Wenjie Zhou、Jianjun Ren、Fuxin Yu、Jingshan Wang、Wujun Ma、Lixian Qiao

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作者单位：

College of Agronomy,Qingdao Agricultural University,Qingdao 266109,Shandong,China

Murdoch University,Food Futures Institute,Perth,WA 6150,Australia

Academy of Dongying Efficient Agricultural Technology and Industry on Saline and Alkaline Land in Collaboration with Qingdao Agricultural University,Dongying 257347,Shandong,China

The Characteristic Laboratory of Crop Germplasm Innovation and Application,Provincial Department of Education,College of Agronomy,Qingdao Agricultural University,Qingdao 266109,Shandong,China

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基金：

National Natural Science Foundation of ChinaQingdao Science & Technology Key ProjectsQingdao Science & Technology Key ProjectsSalt-Alkali Agriculture Industry System of Shandong ProvinceScience & Technology Specific Projects in Agricultural Hightech Industrial Demonstration Area of the Yellow River Delta

项目编号：

3200157822-1-3-1-zyydnsh23-1-3-8-zyydnshSDAIT-29-032022SZX19

出版年：

2024

DOI：

10.1016/j.cj.2024.03.003

作物学报(英文版)

CSTPCD

ISSN：

年,卷(期)：2024.12(3)

参考文献量3