A rice exocyst subunit gene SEC3A is required for anther dehiscence

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外文摘要：Anther dehiscence controls optimal interaction between pollen and stigma,thereby determining the suc-cessful sexual reproduction.The regulators or mechanisms of this process remain elusive.Here,two CRISPR/Cas9 mutants of a rice exocyst subunit gene SEC3A,sec3a-1 and sec3a-2,showed anther indehis-cence at anthesis and male sterility at maturity.Pollen viability and germination in the mutants were partly defective,whereas their female gametes undergone a normal development.Hybrid or self-pollinated seeds could be produced by artificial pollination,suggesting potential use of a weak sec3a mutant as a female line during hybrid breeding.SEC3A is widely expressed in various tissues,including anther walls.Further results showed an excessive IAA accumulation and no endothecium lignification in sec3a-1/2 anthers.Our findings suggest that SEC3A appears to regulate anther dehiscence by modulating auxin signaling,providing insights into regulation of anther dehiscence and function of exocyst in plants.

外文关键词：

RiceMale sterilityAnther dehiscenceExocystSEC3A

作者：

Ranran Tu、Hong Wang、Zhihao Sun、Qinwen Zou、Jiajun Wu、Duo Wu、Nan Wang、Guanghua He

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作者单位：

Rice Research Institute,Key Laboratory of Application and Safety Control of Genetically Modified Crops,Academy of Agricultural Sciences,Southwest University,Chongqing 400715,China

基金：

Science Fund for Creative Research Groups of the National Natural Science Foundation of ChinaChongqing Outstanding Scientists ProjectNatural Science Foundation of Chongqing,China

项目编号：

32171970cstc2022ycjhbgzxm0073cstc2021jcyjcxttX0004

出版年：

2024

DOI：

10.1016/j.cj.2024.04.010

作物学报(英文版)

CSTPCD

ISSN：

年,卷(期)：2024.12(3)