首页|A 9.5-kb deletion in the 1st intron of OsMADS51 enhances temperature sensitivity in rice

A 9.5-kb deletion in the 1st intron of OsMADS51 enhances temperature sensitivity in rice

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Temperature is an important environmental factor affecting heading date of rice.Despite its importance,genes responsible for temperature-sensitive heading in rice have remained elusive.Our previous study identified a quantitative trait locus qHd1 which advances heading date under high temperatures.A 9.5-kb insertion was found in the first intron of OsMADS51 in indica variety Zhenshan 97(ZS97).However,the function of this natural variant in controlling temperature sensitivity has not been verified.In this study,we used CRISPR/Cas9 to knock out the 9.5-kb insertion in ZS97.Experiments conducted under cotrolled conditions in phytotrons confirmed that deletion increased temperature sensitivity and advanced heading by downregulating the expression level of OsMADS51.One-hybrid assays in yeast,ChIP-quantitative polymerase chain reaction,electrophoretic mobility shift,and luciferase-based tran-sient transactivation assays collectively confirmed that OsMADS51 affects heading date by regulation of heading date gene Ehd1.We further determined that the long non-coding RNA HEATINR is generated from the first intron of OsMADS51,offering an explanation for how the 9.5-kb insertion affects temperature sensitivity.We also found that OsMADS51 was strongly selected in early/late-season rice varieties in South China,possibly accounting for their strong temperature sensitivity.These insights not only advance our understanding of the molecular mechanisms underlying the temperature-responsive regulation of heading date in rice but also provide a valuable genetic target for molecular breeding.

OsMADS51InsertionLong non-coding RNAOryza sativa

Lei Zhao、Haifei Hu、Junyu Chen、Chongrong Wang、Yibo Chen、Hong Li、Daoqiang Huang、Zhidong Wang、Degui Zhou、Rong Gong、Yangyang Pan、Junliang Zhao、Liangyong Ma、Shaochuan Zhou

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Rice Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,Guangdong,China

Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510640,Guangdong,China

Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China(Co-construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Guangzhou 510640,Guangdong,China

Guangdong Rice Engineering Laboratory,Guangzhou 510640,Guangdong,China

Guangdong Key Laboratory of New Technology in Rice Breeding

State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement,China National Rice Research Institute,Hangzhou 310006,Zhejiang,China

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Laboratory of Lingnan Modern Agriculture ProjectGuangdong Province Basic and Applied Basic Research Fund-Provincial and Municipal Joint Fund Project广州市科技计划Guangdong Province Rural Revitalization Strategy Special Fund Seed Industry Revitalization Project浙江省自然科学基金Key Laboratory of New Rice Breeding Technologies in Guangdong Province

NT20210012023A15151108822023A04J08112022-NPY-00-013LY22C1300062023B1212060042

2024

10.1016/j.cj.2024.05.010

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(4)
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