首页|The transcriptional landscape of the developmental switch from regular pollen maturation towards microspore-derived plant regeneration in barley

The transcriptional landscape of the developmental switch from regular pollen maturation towards microspore-derived plant regeneration in barley

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Plant formation from in vitro-cultivated microspores involves a complex network of internal and environ-mental factors.Haploids/doubled haploids(DHs)derived from in vitro-cultured microspores are widely used in plant breeding and genetic engineering.However,the mechanism underlying the developmental switch from regular pollen maturation towards microspore-derived plant regeneration remains poorly defined.Here,RNA-sequencing was employed to elucidate the transcriptional landscapes of four early stages of microspore embryogenesis(ME)in barley cultivars Golden Promise and Igri,which exhibit con-trasting responsiveness to microspore-derived plant formation.Our experiments revealed fundamental regulatory networks,specific groups of genes,and transcription factor(TF)families potentially regulating the developmental switch.We identified a set of candidate genes crucial for genotype-dependent respon-siveness/recalcitrance to ME.Our high-resolution temporal transcriptome atlas provides an important resource for future functional studies on the genetic control of microspore developmental transition.

Hordeum vulgareMarker geneMicrospore embryogenesisStress responseTranscriptome analysis

Anna Nowicka、Martin Kovacik、Anna Maksylewicz、Przemys?aw Kope?、Ewa Dubas、Monika Krzewska、Agnieszka Springer、Robert E.Hoffie、Diaaeldin S.Daghma、Zbyněk Milec、Ales Pecinka、Jochen Kumlehn、Iwona ?ur

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The Franciszek Górski Institute of Plant Physiology Polish Academy of Sciences,Niezapominajek 21,30-239 Kraków,Poland

Institute of Experimental Botany of the Czech Academy of Sciences,Centre of Plant Structural and Functional Genomics,Šlechtitel(u)31,779 00 Olomouc,Czech Republic

Department of Cell Biology and Genetics,Faculty of Science,Palacky University Šlechtitel(u) 27,779 00 Olomouc,Czech Republic

Malopolska Centre of Biotechnology,Jagiellonian University,30-387 Kraków,Poland

Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Gatersleben,Corrensstraβe 3,06466 Stadt Seeland,Germany

Institute for Breeding Research on Horticultural Crops,Julius Kühn Institute(JKI)-Federal Research Centre for Cultivated Plants,Erwin-Baur Straβe 27,06484 Quedlinburg,Germany

Global Change Research Institute Czech Academy of Sciences,Bělidla 986/4a,603 00 Brno,Czech Republic

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National Science Center in Poland GrantAnna Nowicka Martin Kovacik and Ales Pecinka were supported by Czech Science Foundation GrantAles Pecinka and European Regional Development Fund project TANGENCZbyněk Milec was funded by AdAgriF

2015/18/M/NZ3/0034821-02929SCZ.02.01.01/00/22_008/0004581CZ.02.01.01/00/22_008/0004635

2024

10.1016/j.cj.2024.07.003

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(4)
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