CRISPR/CasRx-mediated resistance to Soybean mosaic virus in soybean

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外文摘要：Soybean mosaic virus(SMV),an RNA virus,is the most common and destructive pathogenic virus in soy-bean fields.The newly developed CRISPR/Cas immune system has provided a novel strategy for improv-ing plant resistance to viruses;hence,this study aimed to engineer SMV resistance in soybean using this system.Specifically,multiple sgRNAs were designed to target positive-and/or negative-sense strands of the SMV HC-Pro gene.Subsequently,the corresponding CRISPR/CasRx vectors were constructed and transformed into soybeans.After inoculation with SMV,39.02％,35.77％,and 18.70％of T1 plants were confirmed to be highly resistant(HR),resistant(R),and mildly resistant(MR)to SMV,respectively,whereas only 6.50％were identified as susceptible(S).Additionally,qRT-PCR and DAS-ELISA showed that,both at 15 and 30 d post-inoculation(dpi),SMV accumulation significantly decreased or was even unde-tectable in HR and R plants,followed by MR and S plants.Additionally,the expression level of the CasRx gene varied in almost all T1 plants with different resistance level,both at 15 and 30 dpi.Furthermore,when SMV resistance was evaluated in the T2 generation,the results were similar to those recorded for the T1 generation.These findings provide new insights into the application of the CRISPR/CasRx sys-tem for soybean improvement and offer a promising alternative strategy for breeding for resistance to biotic stress that will contribute to the development of SMV-immune soybean germplasm to accelerate progress towards greater soybean crop productivity.

外文关键词：

SoybeanRNA virusSoybean mosaic virusResistanceCRISPR/CasRx

作者：

Le Gao、Lijun Xie、Yanmin Xiao、Xinge Cheng、Ruosi Pu、Ziheng Zhang、Yu Liu、Shaopei Gao、Zilong Zhang、Haoran Qu、Haijian Zhi、Kai Li

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作者单位：

MARA Key Laboratory of Biology and Genetics Improvement of Soybean,Zhongshan Biological Breeding Laboratory(ZSBBL),National Innovation Platform for Soybean Breeding and Industry-Education Integration,State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China

Department of Horticulture,Beijing Vocational College of Agriculture,Beijing 102442,China

College of Bioscience and Biotechnology,Shenyang Agricultural University,Shenyang 110866,Liaoning,China

College of Agronomy & Biotechnology,China Agricultural University,Beijing 100193,China

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基金：

国家自然科学基金Research and Development Program of Beijing Municipal Education CommissionResearch and Development Program of Beijing Municipal Education CommissionScience and Technology Innovation Project of Beijing Vocational College of AgricultureZhongshan Biological Breeding LaboratoryChina Agriculture Research System of MOF and MARAJiangsu Collaborative Innovation Center for Modern Crop Production(JCIC-MCP)Collaborative Innovation Center for Modern Crop Production cosponsored by Province and Ministry(CIC-MCP)

项目编号：

32001571KM202212448003KM202312448004XY-YF-22-02ZSBBL-KY2023-03CARS-04

出版年：

2024

DOI：

10.1016/j.cj.2024.07.007

作物学报(英文版)

CSTPCD

ISSN：

年,卷(期)：2024.12(4)