首页|BnaC03.BIN2 regulates plant height by affecting the main inflorescence length and first effective branch height in Brassica napus L.

BnaC03.BIN2 regulates plant height by affecting the main inflorescence length and first effective branch height in Brassica napus L.

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Rapeseed(Brassica napus L.)is one of the main oil crops in the world,and increasing its yield is of great significance for ensuring the safety of edible oil.Presently,improving rapeseed plant architecture is an effective way to increase rapeseed yield with higher planting density.However,the regulatory mecha-nism of rapeseed plant architecture is poorly understood.In this study,a dwarf rapeseed mutant dwarf08(df08)is obtained by ethyl methane sulfonate(EMS)-mutagenesis.The decrease in plant height of df08 is mainly caused by the reduction in main inflorescence length and first effective branch height and con-trolled by a single semi-dominant gene.The hybrid plants(F1)show a semi-dwarf phenotype.Through map-based cloning and transgenic assay,we confirm that the nonsynonymous single nucleotide variant(SNV)(C to T)in BnaC03.BIN2,which is homologous with Arabidopsis(Arabidopsis thaliana)BIN2,is responsible for the dwarfism of df08.BnaC03.BIN2 interacts with BnaBZR1/BES1 and involves in brassi-nosteroids(BRs)signal transduction.Proline to Leucine substitution in 284(P284L)enhances the protein stability of BnaC03.bin2-D,disrupts BRs signal transduction and affects the expression of genes regulating cell division,leading to dwarfism of df08.This study provides a new insight for the mechanism of rape-seed plant height regulation and creates an elite germplasm that can be used for genetic improvement of rapeseed architecture.

Brassica napusPlant heightBrassinosteroidsBIN2

Chengke Pang、Jun Yu、Liang Zhang、Min Tang、Hongfang Liu、Ying Cai、Feng Chen、Jiefu Zhang、Wei Hua、Xiaodong Wang、Ming Zheng

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Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs,Wuhan 430062,Hubei,China

National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,Hubei,China

Key Laboratory of Cotton and Rapeseed,Ministry of Agriculture and Rural Affairs,Key Laboratory of Jiangsu Province for Agrobiology,Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Jiangsu,China

国家重点研发计划国家自然科学基金国家自然科学基金Central Public-Interest Scientific Institution Basal Research Fund

2022YFD1200401U22A2047732172095Y2022QC21

2024

10.1016/j.cj.2024.05.005

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(4)
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