首页|Fine mapping of two recessive genes TaFLA1 and TaSPL8 controlling flag leaf angle in bread wheat
Fine mapping of two recessive genes TaFLA1 and TaSPL8 controlling flag leaf angle in bread wheat
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
Flag leaf angle is one of the key target traits in high yield wheat breeding.A smaller flag leaf angle reduces shading and enables plants to grow at a higher density,which increases yield.Here we identified a mutant,je0407,with an 84.34％-89.35％smaller flag leaf angle compared with the wild type.The mutant also had an abnormal lamina joint and no ligule or auricle.Genetic analysis indicated that the ligule was controlled by two recessive genes,which were mapped to chromosomes 2AS and 2DL.The mutant allele on chromosome 2AS was named Tafla1b,and it was fine mapped to a 1 Mb physical interval.The mutant allele on chr.2DL was identified as Taspl8b,a novel allele of TaSPL8 with a missense mutation in the sec-ond exon,which was used to develop a cleaved amplified polymorphic sequence marker.F3 and F4 lines derived from crosses between Jing411 and je0407 were genotyped to investigate interactions between the Tafla1b and Taspl8b alleles.Plants with the Tafla1b/Taspl8a genotype had 58.41％-82.76％smaller flag leaf angles,6.4％-24.9％shorter spikes,and a greater spikelet density(0.382 more spikelets per cm)com-pared with the wild type.Plants with the Tafla1a/Taspl8b genotype had 52.62％-82.24％smaller flag leaf angles and no differences in plant height or spikelet density compared with the wild type.Tafla1b/Taspl8b plants produced erect leaves with an abnormal lamina joint.The two alleles had dosage effects on ligule formation and flag leaf angle,but no significant effect on thousand-grain weight.The mutant alleles pro-vide novel resources for improvement of wheat plant architecture.
College of Agriculture,Yangtze University/MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River(Co-Construction by Ministry and Province),Jingzhou 434000,Hubei,China
State Key Laboratory of Crop Gene Resources and Breeding/National Engineering Laboratory of Crop Molecular Breeding/CAEA Research and Development Centre on Nuclear Technology Applications for Irradiation Mutation Breeding,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,C
State Key Laboratory of Crop Gene Resources and Breeding/National Engineering Laboratory of Crop Molecular Breeding/CAEA Research and Development Centre on Nuclear Technology Applications for Irradiation Mutation Breeding,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China
国家重点研发计划Crop Varietal Improvement and Insect Pests Control by Nuclear Radiation,Innovation Program of Chinese Academy of AgriculturaChina Agriculture Research System
NETL
NSTL
万方数据
