首页|Characterization of a 4.1 Mb inversion harboring the stripe rust resistance gene YR86 on wheat chromosome 2AL

Characterization of a 4.1 Mb inversion harboring the stripe rust resistance gene YR86 on wheat chromosome 2AL

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Wheat cultivar Zhongmai 895 was earlier found to carry YR86 in an 11.6 Mb recombination-suppressed region on chromosome 2AL when crossed with Yangmai 16.To fine-map the YR86 locus,we developed two large F2 populations from crosses Emai 580/Zhongmai 895 and Avocet S/Zhongmai 895.Remarkably,both populations exhibited suppressed recombination in the same 2AL region.Collinearity analysis across Chinese Spring,Aikang 58,and 10+wheat genomes revealed a 4.1 Mb chro-mosomal inversion spanning 708.5-712.6 Mb in the Chinese Spring reference genome.Molecular mark-ers were developed in the breakpoint and were used to assess a wheat cultivar panel,revealing that Chinese Spring,Zhongmai 895,and Jimai 22 shared a common sequence named Invcs,whereas Aikang 58,Yangmai 16,Emai 580,and Avocet S shared the sequence named InvAK58.The inverted configuration explained the suppressed recombination observed in all three bi-parental populations.Normal recombi-nation was observed in a Jimai 22/Zhongmai 895 F2 population,facilitating mapping of YR86 to a genetic interval of 0.15 cM corresponding to 710.27-712.56 Mb falling within the inverted region.Thirty-three high-confidence genes were annotated in the interval using the Chinese Spring reference genome,with six identified as potential candidates for YR86 based on genome and transcriptome analyses.These results will accelerate map-based cloning of YR86 and its deployment in wheat breeding.

Adult-plant resistanceChromosomal inversionPuccinia striiformisTriticum aestivum

Qiang Cao、Zhanwang Zhu、Dengan Xu、Jianhui Wu、Xiaowan Xu、Yan Dong、Yingjie Bian、Fugong Ding、Dehui Zhao、Yang Tu、Ling Wu、Dejun Han、Caixia Lan、Xianchun Xia、Zhonghu He、Yuanfeng Hao

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College of Agronomy,Northwest A&F University,Yangling 712100,Shaanxi,China

State Key Laboratory of Crop Gene Resources and Breeding,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences(CAAS),Beijing 100081,China

Food Crops Institute,Hubei Academy of Agricultural Sciences/Wheat Disease Biology Research Station for Central China,Wuhan 430064,Hubei,China

Crop Research Institute,Sichuan Academy of Agricultural Sciences,Chengdu 610066,Sichuan,China

Hubei Hongshan Laboratory,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,Hubei,China

CIMMYT-China Office,C/O CAAS,Beijing 100081,China

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National Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaAgricultural Science and Technology Innovation Program,Fundamental Research Funds for Central Non-Profit of Institute of CroAgricultural Science and Technology Innovation Program,Fundamental Research Funds for Central Non-Profit of Institute of Cro

2022YFD12009002022YFD1200904232490022A232490432A

2024

10.1016/j.cj.2024.05.011

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(4)
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