首页|Engineering high amylose and resistant starch in maize by CRISPR/Cas9-mediated editing of starch branching enzymes
Engineering high amylose and resistant starch in maize by CRISPR/Cas9-mediated editing of starch branching enzymes
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To improve the amylose content(AC)and resistant starch content(RSC)of maize kernel starch,we employed the CRISPR/Cas9 system to create mutants of starch branching enzyme Ⅰ(SBEⅠ)and starch branching enzyme Ⅱb(SBEⅡb).A frameshift mutation in SBEI(E1,a nucleotide insertion in exon 6)led to plants with higher RSC(1.07％),lower hundred-kernel weight(HKW,24.71±0.14 g),and lower plant height(PH,218.50±9.42 cm)compared to the wild type(WT).Like the WT,E1 kernel starch had irreg-ular,polygonal shapes with sharp edges.A frameshift mutation in SBEⅡb(E2,a four-nucleotide deletion in exon 8)led to higher AC(53.48％)and higher RSC(26.93％)than that for the WT.E2 kernel starch was significantly different from the WT regarding granule morphology,chain length distribution pattern,X-ray diffraction pattern,and thermal characteristics;the starch granules were more irregular in shape and comprised typical B-type crystals.Mutating SBEI and SBEⅡb(E12)had a synergistic effect on RSC,HKW,PH,starch properties,and starch biosynthesis-associated gene expression.SBEⅡa,SS1,SSⅡa,SSⅢa,and SSⅢb were upregulated in E12 endosperm compared to WT endosperm.This study lays the foundation for rapidly improving the starch properties of elite maize lines.
NETL
NSTL
万方数据
