首页|Introgression of lac6/tl1/du13 improves the palatability of japonica rice
Introgression of lac6/tl1/du13 improves the palatability of japonica rice
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Amylose content(AC)is a crucial determinant of the eating and cooking quality(ECQ)of rice,with low AC varieties exhibiting a softer texture and greater stickiness-attributes that enhance palatability and are desirable in specific culinary contexts.To harness these traits,significant efforts have been made to manipulate AC to improve rice ECQ.Our research utilized the MutMap+approach to identify LAC6/TL1,a gene that is an allele of Du13,responsible for low AC.LAC6 encodes a C2H2 zinc finger protein,which specifically increases the splicing efficiency of the Wxb allele without affecting the Wxa allele.Functional studies of LAC6 revealed that its proper integration could rectify the undesirable AC phenotype,whereas mutations within this gene led to reduced AC and were associated with shorter grain length and decreased thousand-grain weight.Despite these drawbacks,such mutations positively impact rice palatability,presenting a trade-off between grain size and eating quality.To address the challenges posed by the reduced grain weight associated with LAC6 mutations,we developed a specific molecular marker for LAC6,which has been effectively used in breeding programs to select lac6/tl1/du13 homozygous indi-viduals with larger grain size.Our findings demonstrate that the"small grain"trait associated with lac6/tl1/du13 can be effectively mitigated through combined phenotype-based and marker-assisted selection.This study highlights the potential of lac6/tl1/du13 as a valuable gene for breeding novel,high-quality soft rice varieties through targeted breeding strategies.
Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education,Agricultural College of Yangzhou University,Yangzhou 225009,Jiangsu,China
Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology,Yangzhou University,Yangzhou 225009,Jiangsu,China
Jiang Su(Wu Jin)Rice Research Institute,Changzhou 213175,Jiangsu,China
国家重点研发计划国家自然科学基金国家自然科学基金Research Programs from Jiangsu Government and Qing Lan ProjectResearch Programs from Jiangsu Government and Qing Lan ProjectForeign Expert ProjectPostgraduate Research & Practice Innovation Program of Jiangsu ProvinceYangzhou University Highend Talent Program
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