Understanding mechanisms for differential salinity tissue tolerance between quinoa and spinach:Zooming on ROS-inducible ion channels

扫码查看

原文链接

万方数据
维普

外文摘要：Soil salinity is a worldwide issue and a major threat to global food security. Salinity tolerance is a complex mechanism that is conferred by numerous molecular,physiological,and biochemical traits. Of critical importance are plant's ability to regulate redox balance without compromising reactive oxygen species (ROS) signalling and maintain cytosolic ion homeostasis. In this study,the mechanistic basis of K+reten-tion ability in leaf mesophyll (an important but highly sensitive plant tissue) was compared between halophytic quinoa and glycophytic spinach. Phenotypic data showed quinoa outperformed spinach under 100 to 500 mmol L-1 NaCl salinity. The major difference behind this differential salinity sensitivity was a differential K+uptake in leaf mesophyll. Electrophysiological and molecular experiments revealed that a superior ability of mesophyll K+retention in quinoa was conferred by three complementary mechanisms:(ⅰ) an intrinsically lower H+-ATPase activity in quinoa (potentially as an energy saving strategy);(ⅱ) reduced sensitivity of K+transporters to ROS;and (ⅲ) increased sensitivity of ROS-inducible Ca2+-permeable channels. Moreover,the sensitivity of K+-transport systems to ROS was further examined in NaCl-acclimated quinoa and spinach plants. The key factors differentiating between K+retention in accli-mated leaf mesophyll was associated with the reduced sensitivity and gene expression of K+-permeable outward rectifying channel (GORK),Arabidopsis potassium transporter 1 (AKT1),and high affinity potas-sium transporter 5 (HAK5) to additional NaCl and ROS stress,along with the upregulation of ROS scaveng-ing system. Taken together,our results showed that the tissue-specific and ROS-specific regulation of K+retention are important for conferring salinity tolerant at least in the halophyte quinoa.

外文关键词：

AcclimationMembrane potentialROS signallingLeaf mesophyllChenopodium quinoaSpinacia oleracea

作者：

Mohsin Tanveer、Lei Wang、Liping Huang、Meixue Zhou、Zhong-Hua Chen、Sergey Shabala

展开 >

作者单位：

State Key Laboratory of Desert and Oasis Ecology,Xinjiang Institute of Ecology and Geography,Chinese Academy of Sciences,Urumqi 830000,Xinjiang,China

International Research Centre for Environmental Membrane Biology,Foshan University,Foshan 528000,Guangdong,China

Tasmanian Institute of Agriculture,University of Tasmania,Hobart,TAS 7005,Australia

School of Science,Western Sydney University,Penrith,NSW 2751,Australia

Hawkesbury Institute for the Environment,Western Sydney University,Penrith,NSW 2751,Australia

School of Biological Sciences,University of Western Australia,Perth,WA 6009,Australia

展开 >

出版年：

2024

DOI：

10.1016/j.cj.2024.03.001

作物学报(英文版)

CSTPCD

ISSN：

年,卷(期)：2024.12(5)