CRISPR-Cas9-mediated promoter editing of FERONIA-Like receptor 13 increases plant growth and disease resistance in rice

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外文摘要：Receptor kinases play a pivotal role in detecting environmental signals,and consequently,gene pleio-tropy is frequently observed within this family.However,the trade-off in trait expression resulting from gene pleiotropy poses a constraint on the utilization of such genes in agricultural breeding.In this study,we identified the receptor kinase gene FERONIA-Like Receptor 13(FLR13)as a pleiotropic gene influencing plant height,tillering,grain yield,and disease resistance.Using promoter editing,we generated novel alleles(FLR13T5T6-1,FLR13T5T6-2)that confer resistance to rice blast and increase per-plant yield.The knockout of the T5T6 segment alleviates the inhibitory effects of two transcription factors,OsGBP1 and OsWRKY53,on FLR13 expression.In summary,our study presents a promising avenue for enhancing the pivotal attributes of receptor-like kinases through a promoter-editing strategy.

外文关键词：

Oryza sativa L.FERONIA like receptorCRISPR-Cas9-mediated promoter editingPlant growthResistanceOsWRKY53

作者：

Yanan Guo、Yinyao Qi、Kai Liu、Xiao Luo、Weiyu Xiao、Xiaonan Qiang、Junjie Xing、Feng Yu、Zhenghong Zhao、Long Wang、Lifeng Wang

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作者单位：

Longping Branch,College of Biology,Hunan University,Changsha 410125,Hunan,China

College of Biology,Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation,Hunan University,Changsha 410082,Hunan,China

Hunan Rice Research Institute,Hunan Academy of Agricultural Science,Changsha 410015,Hunan,China

State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Hunan Academy of Agricultural Sciences,Changsha 410015,Hunan,China

Huangpu Research Institute of Longping Agricultural Science and Technology,Guangzhou 510700,Guangdong,China

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出版年：

2024

DOI：

10.1016/j.cj.2024.09.019

作物学报(英文版)

CSTPCD

ISSN：

年,卷(期)：2024.12(6)