首页|Gene editing and overexpression of soybean miR396a reveals its role in salinity tolerance and development

Gene editing and overexpression of soybean miR396a reveals its role in salinity tolerance and development

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MicroRNAs(miRNAs)are versatile regulators of gene expression at both the transcription and post-transcription levels.The microRNA miR396 plays vital roles in growth,development,and resistance to abiotic stresses in many plant species.However,the roles and functions of miR396 in soybeans are not well understood.Here,we report that Gm-miR396a influences soybean development and salinity toler-ance.We found that soybean miR396a was responsive to salt stress.Gm-miR396a gene-edited lines(miR396a-GEs),created using CRISPR/Cas9,exhibited more branches,higher grain yields,and greater salinity tolerance than control plants.The transcripts in lines with altered abundance of miR396a-GE were significantly enriched for biological processes related to hormone regulation.Overexpression of the Gm-miR396a precursor(pre-miR396a-OE)resulted in developmental deficiencies including dwarf-ness,abnormal inflorescences and flowers,smaller and fewer seeds,and small leaves with larger and more numerous stomata.Transcriptome analysis indicated photosynthesis-related genes were downreg-ulated in pre-miR396a-OE plants.These results contribute valuable insights into the function of Gm-miR396a in soybeans and hold promise for enhancing soybean yield and salinity tolerance through germ-plasm innovation.

Glycine maxGm-miR396aSalt stressCRISPR/Cas9

Xiangqian Chen、Xuemin Jiang、Xianjun Sun、Zheng Hu、Fei Gao、Xiuping Wang、Hui Zhang、Rui Chen、Qiyan Jiang

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State Key Laboratory of Crop Gene Resources and Breeding,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

Key Laboratory of Grain Crop Genetic Resources Evaluation and Utlization Ministry of Agriculture and Rural Affairs,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

College of Life and Environmental Sciences,Minzu University of China,Beijing 100081,China

Institute of Coastal Agriculture,Hebei Academy of Agriculture and Forestry Science,Tangshan 063299,Hebei,China

Institute of Crop Germplasm and Biotechnology,Tianjin Academy of Agricultural Sciences,Tianjin 300192,China

National Nanfan Research Institute,Chinese Academy of Agricultural Sciences,Sanya 572000,Hainan,China

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2024

10.1016/j.cj.2024.08.003

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(6)