首页|A natural allelic variant of GmSW17.1 confers high 100-seed weight in soybean

A natural allelic variant of GmSW17.1 confers high 100-seed weight in soybean

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Soybean(Glycine max)is an important and valuable crop,providing oil and proteins for both humans and animals.Seed weight is a key trait that determines soybean yields;however,the genes and mechanisms controlling seed weight remain poorly understood.Here,we used genome-wide association study(GWAS)and joint linkage mapping to identify a ubiquitin-specific protease,GmSW17.1,which regulates 100-seed weight in soybean.Two natural allelic variants of GmSW1 7.1 resulted in significantly different 100-seed weight,with GmSW17.1T conferring heavier seeds.We used CRISPR/Cas9 technology to knock out GmSW17.1,resulting in lighter and smaller seeds;however,these mutants produced more seeds than the wild type,resulting in similar overall yields.Owing to the increased number of seeds,we determined that GmSW17.1 is highly transcribed in developing seeds,and its encoded protein physically interacts in the nucleus with GmSGF1 1,which plays a crucial role in the deubiquitinating pathway.Analysis of geno-mic sequences from more than 1714 soybean accessions suggested that the natural allele GmSW17.1T was selected during the domestication and genetic improvement,resulting in its rapid expansion in cultivated soybean.These findings provide important insights into the role of GmSW1 7.1 in 100-seed weight and offer valuable clues for the molecular breeding of soybean.

GWASGmSW17.1Natural allelic variant100-Seed weightSoybean

Hao Zhang、Lei Yang、Shiyu Guo、Yu Tian、Chunyan Yang、Chaosen Zhao、Zhihui Shan、Qi Wang、Zihao Zheng、Ying-hui Li、Li-juan Qiu

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State Key Laboratory of Crop Gene Resources and Breeding/The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization(MARA),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang Branch Center of National Center for Soybean Improvement,The Key Laboratory of Crop Genetics and Breeding,Shijiazhuang 050031,Hebei,China

Crops Research Institute of Jiangxi Academy of Agricultural Sciences/Nanchang Branch of National Centre of Oil Crops Improvement/Jiangxi Province Key Laboratory of Oil Crops Biology,Nanchang 330200,Jiangxi,China

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062,Hubei,China

Department of Agronomy,Iowa State University,Ames,IA 50011-1051,USA

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2024

10.1016/j.cj.2024.10.004

作物学报(英文版)

作物学报(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.12(6)