首页|Characterization of novel wheat-Thinopyrum ponticum introgression lines derived from partial amphiploid AUS6770 for resistance to stripe rust
Characterization of novel wheat-Thinopyrum ponticum introgression lines derived from partial amphiploid AUS6770 for resistance to stripe rust
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万方数据
维普
The wild decaploid species Thinopyrum ponticum(Podp.)Barkworth&D.R.Dewey is an important source of genes against biotic and abiotic stresses affecting wheat.The wheat-Th.ponticum partial amphiploid AUS6770 shows resistance to multiple diseases,including stripe rust,stem rust,and powdery mildew.Mitotic chromosomes of AUS6770 were characterized by non-denaturing-fluorescence in situ hybridiza-tion(ND-FISH),and the individual Th.ponticum chromosomes 1Ae to 7Ae were karyotypically distin-guished by Oligo-FISH painting using bulked oligo pools based on wheat-barley collinear regions.A novel stripe rust resistant line A1 55,derived from AUS6770,was found to have 44 chromosomes,includ-ing a pair of 2Ae chromosomes and a pair of 6B-6Ae translocations.To detect plants with transfer of resis-tance genes from A1 55 to wheat chromosomes,1770 plants were developed from F2-F5 progenies of A1 55 crossed with the susceptible wheat cultivar MY11 and characterized with ND-FISH using multiple probes.A high frequency of transmission of chromosome 2Ae was observed,and 31 types of 2Ae chromo-somal aberrations were identified using ND-FISH.Ten chromosomal bins on the 2Ae chromosome were determined from the deletion and translocation lines based on genome-based PCR markers.In combina-tion with the evaluation of disease resistance,the gene(s)for stripe rust resistance was located on the FL0.79-1.00 of 2AeS and covers the corresponding region of 0-58.26 Mb in the reference genome of Th.elongatum.The newly identified wheat-Th.ponticum 2Ae translocation lines can be exploited as poten-tial germplasm in wheat breeding for stripe rust resistance.
万方数据
维普
