首页|Fine mapping of a major QTL on chromosome A05 conferring pod size in peanut
Fine mapping of a major QTL on chromosome A05 conferring pod size in peanut
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维普
Pod size is a key agronomic trait that influences peanut yield greatly.However,our understanding of the mechanisms underlying pod size is limited.In this study,we employed a segregating population derived from a cross between the small-pod line ND_S and the large-pod line ND_L to map quantitative trait loci(QTL)associated with pod size.Initial mapping performed using bulk segregant analysis revealed a can-didate interval on chromosome A05 referred to as qPSW05.We refined this interval to a 256.9 kb genomic region using newly developed molecular markers.Through sequence and expression analyses,we iden-tified the candidate gene AhXE45GC,which encodes an AN1 zinc finger protein.We discovered a 33-bp insertion in the intron of AhXE45GC in ND_S.Accessions that lack this insertion,such as ND_L,had signif-icantly larger pods than those with the insertion,including ND_S.To facilitate marker-assisted selection for peanut pod size,we developed a molecular marker associated with this polymorphism.This marker could provide a valuable genetic resource for breeding high-yielding peanut varieties.
万方数据
维普
